文献类型：期刊文献

中文题名：在无血清培养基中代谢副产物对杂交瘤细胞生长代谢的影响I.乳酸的作用

英文题名：Influence of Metabolic Byproducts on Growth and Metabolism of Hybridoma Cells in Serum-free Cultures I. Effect of Lactate

作者：张立[1];金亦辉[1];张元兴[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2002

卷号：28

期号：2

起止页码：157

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：2002397116936);Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家九五重点科技攻关项目 (95 0 2 0 3 0 5 )

语种：中文

中文关键词：杂交瘤细胞;细胞培养;乳酸;无血清培养

外文关键词：lactate; hybridoma cell; cell culture; serum free culture

摘要：研究了无血清批培养中乳酸对杂交瘤细胞生长代谢的影响。当乳酸浓度低于 5 .3mmol/L时 ,乳酸对细胞生长和代谢无明显影响 ;当乳酸浓度在 1 0 mmol/L～ 2 5 mmol/L时 ,对杂交瘤细胞的生长代谢有弱抑制 ;当乳酸浓度高于 34mmol/L时 。
In the batch cultures of WuT3 hybridoma cells in serum free medium with different lactate concentrations, the influences of lactate on cells growth and metabolism were investigated. It was found that lactate below 5.3mmol/L influenced hardly on cells, lactate between 10mmol/L and 25mmol/L inhibited a little on cells growth and metabolism, and lactate above 34mmol/L inhibited strongly on cell cultures.