文献类型：期刊文献

英文题名：Novel Engineered Living Materials for Integrated Detection, Remediation, and Recovery of Cadmium Pollution

作者：Chen, Jianshu[1];Zhu, Xiaojuan[1,2,3];Yang, Lixin[2];Qi, Tong[4];Liang, Lijuan[4];Huang, Qungang[5];Yan, Riming[2];Zhu, Du[2];Jiang, Ning[1];Yang, Sainan[1];Zhuang, Yingping[1];Liang, Rubing[6];Zhang, Xuehong[6];Zhao, Liming[1];Huang, Jiaofang[1,2]

机构：[1]East China Univ Sci & Technol ECUST, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Jiangxi Normal Univ JXNU, Coll Life Sci, Nanchang 330022, Peoples R China;[3]Guizhou Med Univ, Coll Basic Med, Guiyang, Guizhou, Peoples R China;[4]Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, Shanghai, Peoples R China;[5]Jiangxi Brother Pharmaceut Co Ltd, Brother Res Ctr, Jiujiang 332700, Peoples R China;[6]Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai, Peoples R China

年份：2026

外文期刊名：ADVANCED FUNCTIONAL MATERIALS

收录：;EI(收录号：20263121226961);Scopus(收录号：2-s2.0-105046296168);WOS:【SCI-EXPANDED(收录号:WOS:001836584100001)】；

基金：This work was supported by the Science and Technology Commission of Shanghai Municipality (23HC1400302), the Open Funding Project of State Key Laboratory of Microbial Metabolism (No: MMLKF24-01), and the Jiangxi Provincial Natural Science Foundation (No: 20262BAC260022). We thank the Research Center of Analysis and Test (ECUST) for the assistance provided, in particular engineers L.F. Zhang and Y.Q. Xi for supplying the ICP-OES data. We thank Professor Fener Chen for his constructive comments of the paper.

语种：英文

外文关键词：biosensor; Cd2+Bioremediation; CdS NPs; engineered living materials; magnetic recovery

摘要：Heavy-metal emissions pose severe environmental hazards, with Cd2+ contamination being particularly critical. However, current strategies for Cd2+ detection and remediation remain limited and often inflict secondary ecological damage. Here, we engineered Bacillus subtilis as a chassis to construct a Cd2+-responsive biosensor (CJSora) by integrating the endogenous promoter of cadA (pcadA) with a T7RNAP-based signal amplification module. The resulting sensor enables monitoring of environmental Cd2+ with high specificity and sensitivity across a 0-100 & micro;M range. To achieve intelligent detection and remediation, we further developed an engineered living material (CJSsorX) capable of autonomous response to Cd2+ pollution. Under low Cd2+ conditions (e.g., agricultural soils), surface-displayed Cd2+-binding protein CadR efficiently captures and recovers Cd2+ from the environment. When Cd2+ levels exceed the adsorption capacity, intracellular overexpression of the aerobic sulfate reduction pathway (CysE and PatB) catalyzes the biomineralization of Cd2+ into CdS nanoparticles, enabling spatially modular remediation with enhanced efficiency and sustainability. After remediation, Cd2+ can be reclaimed using a functional peptide (TVNFKLY) with specific affinity for Fe3O4 magnetic nanoparticles. As a proof of concept, CJSsorX was applied to the removal of Cd2+ from contaminated soils and wastewater. The engineered material achieved 83.64% removal in soils and up to 99.56% removal in water. Overall, this work establishes a B. subtilis-based intelligent living material with integrated sensing, remediation, and recovery capabilities, offering a promising and sustainable strategy for combating Cd2+ pollution.