文献类型：期刊文献

英文题名：Micropatterned Protein for Cell Adhesion through Phototriggered Charge Change in a Polyvinylpyrrolidone Hydrogel

作者：Ming, Zunzhen[1];Ruan, Xing[1];Bao, Chunyan[1];Lin, Qiuning[1];Yang, Yi[2];Zhu, Linyong[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Inst Fine Chem, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2017

卷号：27

期号：25

外文期刊名：ADVANCED FUNCTIONAL MATERIALS

收录：;EI(收录号：20172003668111);WOS:【SCI-EXPANDED(收录号:WOS:000404550600001)】；

基金：This work was supported by the NSFC (Grant Nos. 21472044, 51273064, 21425311, and 51403061), and the the program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning.

语种：英文

外文关键词：cell adhesion; electrostatic interactions; hydrogels; photoregulation; protein immobilization

摘要：Regulated immobilization of proteins on hydrogels allows for the creation of highly controlled microenvironments to meet the special requirements of cell biology and tissue engineering devices. Light is an ideal stimulus to regulate immobilization because it can be controlled in time, space, and intensity. Here, a photoresponsive hydrogel that enables the patterning of proteins by a combination of electrostatic adsorption and photoregulated charge change on a hydrogel is developed. It is based on a photosensitive cationic monomer (CLA), a coumarin caged lysine betaine zwitterion, incorporated into a poly-vinylpyrrolidone (PVP) hydrogel, which can controllably change the charge from an adhesive positive state to an anti-adhesive zwitterion state upon irradiation at 365 nm. With this strategy, the immobilization of proteins is regulated and cell adhesion is programmed on hydrogels on demand. This approach should open up new avenues for hydrogels in biomedical applications.