文献类型：期刊文献

中文题名：一种复合二氧化钛大孔共聚物的合成及其在扩张床吸附中的应用(Ⅱ) 扩张和流体混合性能以及吸附性能

英文题名：PREPARATION AND PROPERTIES OF COMPOSITE TiO_2-MACROPOROUS COPOLYMER AND ITS APPLICATION FOR EXPANDED BED ADSORPTION (Ⅱ) EXPANSION CHARACTERISTICS,LIQUID MIXING AND ADSORPTION PERFORMANCE

作者：钱建华[1];雷引林[2];刘坐镇[1];姚善泾[2];邬行彦[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]浙江大学宁波理工学院,浙江宁波315100

年份：2004

卷号：55

期号：1

起止页码：9

中文期刊名：化工学报

外文期刊名：CIESC Journal

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：扩张床;吸附;牛血清白蛋白

外文关键词：expanded bed,adsorption,BSA

摘要：利用GET共聚物骨架结构中的环氧基与二乙胺反应得到的阴离子交换层析介质DEAE GET ,考察了该介质在扩张床STREAMLINE 2 5中不同黏度和流速对扩张性能和流体混合性能的影响 ,结果表明DEAE GET在STREAMLINE 2 5中的扩张行为服从Richardson Zaki方程 .在研究的范围内 ,随着流体黏度和流速 (10 0～ 5 0 0cm·h-1)的增加 ,理论塔板数和Bodenstein数逐渐减小 ,轴向混合系数逐渐增加 ;扩张床中的轴向混合系数很小 (10 -6～ 10 -5m2 ·s-1) ,表明柱内流体以近似平推流流动 .在静态条件下DEAE GET对牛血清白蛋白 (BSA)的饱和吸附量为 4 3 0mg·ml-1.同时比较了DEAE GET与STREAMLINEDEAE分别在固定床和扩张床中对BSA的穿透曲线 ,发现都比较接近 ,DEAE GET在固定床和扩张床中对BSA的最大吸附量分别为 2 8 0mg·ml-1和 2 6 2mg·ml-1.
In the first part of this paper, the GET composite copolymer with pendant epoxy groups were prepared. It was reacted with diethylamine, forming an anion-exchange chromatographic medium DEAE-GET. The effects of liquid viscosity, flow velocity on expansion characteristics and liquid mixing performance of DEAE-GET in expanded bed were studied. Its expansion characteristics in STREAMLINE 25 column were found to obey the Richardson-Zaki equation. The increase of liquid viscosity, flow velocity resulted in the increase of axial mixing coefficient. In the range of flow velocity 100—500cm·h -1 , the low value of axial mixing coefficient in expanded bed (10 -6 —10 -5 m 2·s -1 ) indicated that the liquid flow through the expanded bed was close to a plug flow profile. The maximum static adsorption capacity for BSA was found to be 43.0mg·ml -1 .The breakthrough curves for BSA adsorption on DEAE-GET and STREAMLINE DEAE in packed bed*'and*'expanded*'bed*'were*'compared,*'showing*'for*'each*'adsorbent*'they*'were*'close*'to*'each*'other.*'The*'*' maximum*'adsorption capacities on DEAE-GET in packed bed and expanded bed were 28.0mg·ml -1 and 26.2mg·ml -1 respectively.