文献类型：期刊文献

英文题名：A Type of Ferrocene-Based Derivative FE-1 COF Material for Glycopeptide and Phosphopeptide Selective Enrichment

作者：Wu, Yu[1];Xu, Sen[1];Ding, Fengjuan[2];Zhang, Weibing[1];Liu, Haiyan[2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chempistry & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China

年份：2024

卷号：15

期号：7

外文期刊名：JOURNAL OF FUNCTIONAL BIOMATERIALS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001277449000001)】；

基金：We gratefully acknowledged the support of the National Natural Science Foundation of China (No. 21974045), the Science and Technology Commission of Shanghai Municipality(No. 19142201100, 6142200500), the Fundamental Research Funds for the Central Universities(No. 50321102117022).

语种：英文

外文关键词：covalent organic framework materials; simultaneously enrichment; phosphopeptides; glycopeptides

摘要：In this work, a new type of FE-1 COF material is prepared by a reversible imine condensation reaction with diaminoferrocene and diaminodiformaldehyde as materials. The material is connected by imine bonds to form a COF skeleton, and the presence of plenty of nitrogen-containing groups gives the material good hydrophilicity; the presence of metal Fe ions provides the material application potential in the enrichment of phosphopeptides. According to the different binding abilities of N-glycopeptide and phosphopeptide on FE-1 COF, it can simultaneously enrich N-glycopeptide and phosphopeptide through different elution conditions to realize its controllable and selective enrichment. Using the above characteristics, 18 phosphopeptides were detected from alpha-casein hydrolysate, 8 phosphopeptides were detected from beta-casein hydrolysate and 21 glycopeptides were detected from IgG hydrolysate. Finally, the gradual elution strategy was used; 16 phosphopeptides and 19 glycopeptides were detected from the alpha-casein hydrolysate and IgG hydrolysate. The corresponding glycopeptides and phosphopeptides were identified from the human serum. It proves that the FE-1 COF material has a good enrichment effect on phosphopeptides and glycopeptides.