文献类型：期刊文献

英文题名：A ΣE-mediated temperature gauge orchestrates type VI secretion system, biofilm formation and cell invasion in pathogen Pseudomonas plecoglossicida

作者：Zhang, Yibei[1,4];Huang, Yuping[1];Ding, Haoyuan[1];Ma, Jiabao[1];Tong, Xinyu[1];Zhang, Yuanxing[1,2,4];Tao, Zhen[3];Wang, Qiyao[1,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China;[3]Zhejiang Ocean Univ, Sch Fisheries, Zhoushan, Peoples R China;[4]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China

年份：2023

卷号：266

外文期刊名：MICROBIOLOGICAL RESEARCH

收录：;EI(收录号：20224413021202);WOS:【SCI-EXPANDED(收录号:WOS:000884844300003)】；

基金：Acknowledgments This work was supported by grants from the National Natural ScienceFoundation of China (32102850) , National Key Research and Develop-ment Program (2022YFE0101200) , Postdoctoral Science Foundation of China (2021M701206) , and Fundamental Research Funds for Zhejiang Provincial Universities and Research Institutes (2021J012) .

语种：英文

外文关键词：Temperature; Sigma factor; T6SS; Pseudomonas plecoglossicida

摘要：Pseudomonas plecoglossicida is a temperature-dependent opportunistic pathogen mediating visceral granulomas in many piscine species including the large yellow croaker (Larimichthys crocea) but the underlying mechanisms are unclear. RpoE is an alternative sigma (sigma) factor involved in regulated intramembrane proteolytic (RIP) cascade, enabling bacterial pathogens to coordinate the expression of genetic traits associated with stress adaptation and virulence determinants in response to diverse stimuli in vitro and in vivo of the hosts. In this study, genes associated to RIP cascade in P. plecoglossicida were identified and characterized to show various sequence sim-ilarities to their counterparts in Escherichia coli and P. aeruginosa. The expression of P. plecoglossicida RIP locus was induced by higher temperatures. Moreover, RNA sequencing approach revealed that RpoE regulated the expression of-297 and-261 genes at virulent (18 degrees C) and non-virulent (28 degrees C) temperatures, respectively. RpoE regulon genes are involved in various processes associated with bacterial signal transduction, membrane homeostasis, energy metabolism and virulence. In particular, RpoE positively controlled expression of csrA encoding an RNA binding protein essential for central carbon metabolism. In addition, P. plecoglossicida RpoE was validated to regulate type VI secretion system (T6SS) expression, bacteria competition, biofilm formation and reproduction in macrophages. Collectively, RpoE-centered RIP cascade appeared to play important roles in control of the expression of genes involved in adaptation in vivo and in vitro niches by thermal sensing in P. plecoglossicida. These results facilitates to reveal the pathogenic mechanisms of P. plecoglossicida causing fish diseases and provides new perspectives to control bacterial infection.