文献类型：期刊文献

中文题名：CRISPR-Cas系统在食源性致病菌中的结构与功能研究进展

英文题名：Research Progress on Structure and Function of CRISPR-Cas Systems in Foodborne Pathogens

作者：王翔[1];卓启云[1];吴瑜凡[2,3];刘阳泰[1];李红梅[1];董庆利[1]

机构：[1]上海理工大学医疗器械与食品学院,上海200093;[2]张家港海关综合技术中心,江苏苏州215600;[3]华东理工大学化学与分子工程学院分析测试中心,上海200237

年份：2021

卷号：21

期号：11

起止页码：329

中文期刊名：中国食品学报

外文期刊名：Journal of Chinese Institute Of Food Science and Technology

收录：CSTPCD;;EI(收录号：20215111333740);Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：江苏省自然科学基金青年科学基金项目(BK20190235);国家自然科学基金青年科学基金项目(31801455)。

语种：中文

中文关键词：规律成簇间隔短回文重复序列;食源性致病菌;耐药性;毒性;生物膜;

外文关键词：CRISPR;foodborne pathogens;drug resistance;virulence;biofilm;

摘要：规律成簇间隔短回文重复序列(CRISPR)及其相关蛋白(Cas)构成的CRISPR-Cas系统是存在于细菌和古细菌中的免疫系统的组成部分,能有效防御外源移动遗传元件,如噬菌体的侵袭。研究发现该系统可以阻止携带特征基因的外源核酸整合到细菌基因组中,同时还有调节其它功能基因表达的作用,对细菌毒性、耐药性、生物膜形成等生物学特征也有影响。本文解析常见食源性致病菌CRISPR-Cas系统的结构,综述CRISPR-Cas系统与细菌毒性、耐药性、生物膜形成、分型等的相关性,为后续CRISPR-Cas系统功能研究以及食源性致病菌的溯源、控制提供参考。
Clustered regularly interspaced short palindromic repeats(CRISPR)system and CRISPR-associated proteins(Cas)make up CRISPR-Cas system which is one part of the immune system exists in bacteria and archaea,and can defend against the invasion of foreign mobile genetic elements such as phages effectively.Studies have found this system can prevent foreign nucleic acids which carrying feature genes integrating into bacterial genome and has effect on regulating functional genes expression,it is essential for biological characteristics such as bacterial virulence,antibiotics resistance,and the ability of biofilm formation.This article analyzed the structure of CRISPR-Cas in the common foodborne pathogens,and reviewed the functional correlation between CRISPR-Cas system and bacterial virulence,antibiotics,biofilm formation and typing,provided a reference for subsequent functional research of CRISPR-Cas system and traceability and control of foodborne pathogens.