文献类型：期刊文献

英文题名：Composition and transcriptional activity of oil reservoir microorganisms under different gas/liquid ratios

作者：Shou, Li-Bin[1,2];Hou, Zhao-Wei[3];Liu, Yi-Fan[1,2];Wang, Ying[3];Zhou, Lei[1,2];Liu, Jin-Feng[1,2];Yang, Shi-Zhong[1,2];Gu, Ji-Dong[4];Wu, Xiao-Lin[3];Mu, Bo-Zhong[1,2,5]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]PetroChina, Explorat & Dev Res Inst Daqing Oilfield Co Ltd, Daqing 163712, Heilongjiang, Peoples R China;[4]Guangdong Technion Israel Inst Technol, Environm Engn, 241 Daxue Rd, Shantou 515063, Guangdong, Peoples R China;[5]East China Univ Sci & Technol, Engn Res Ctr Microbial Enhanced Oil Recovery, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2023

卷号：181

外文期刊名：INTERNATIONAL BIODETERIORATION & BIODEGRADATION

收录：;EI(收录号：20232014088282);WOS:【SCI-EXPANDED(收录号:WOS:001008073200001)】；

基金：Acknowledgments We are grateful to the management of Daqing Oil Field for sampling support. This work was supported by the National Natural Science Foundation of China (Grant No. 42173076, 42061134011 and 52074129) , the Natural Science Foundation of Shanghai (Grant No. 21ZR1417400) , Fundamental Research Funds for the Central Univer-sities (No. JKJ01221716) and the Research Program of State Key Lab-oratory of Bioreactor Engineering to Y.F.L. and B.Z.M, the NSFC/RGC Joint Research Fund (No. 41161160560) to J.D.G.

语种：英文

外文关键词：Gas; liquid ratio; Microbial oil-displacement functions; Microbial community; Transcriptional activity

摘要：Microorganisms inhabited in oil reservoirs play a fundamental role in microbial enhanced oil recovery. Gas injection has been adopted as an alternative method in the tertiary oil recovery, but the influence of the gas injection on microbial composition in oil reservoir and its contribution to oil recovery still remained unclear. In this study, the effect of air injection on the microbial composition and transcriptional activity is investigated using a combination of the 16S rRNA gene amplicon sequencing, metagenomic and meta-transcriptomic analysis. Firmicutes were the main active bacteria in all samples and the proportion of Firmicutes increased with the rising of gas/liquid ratio in samples without additional treatment. The Bacillus was the dominant member of phylum Firmicutes. With the increase of gas-to-liquid ratios, the microbes showed higher overall transcription activities and propionate become an important metabolite. This research provides theoretical guidance for the activation of endogenous oil displacement microorganisms and sheds light on the industrial applications for exploitation of depleted reservoirs and the extension of the development life of oil reservoirs.