文献类型：期刊文献

英文题名：Selective visualization of live-cell mitochondria' thiophenols and their induced oxidative stress process by a rationally designed rhodol-based fluorescent probe

作者：Liu, Qimeng[1];Li, Anyang[1];Li, Xiaokang[2];Li, Bin[3];Zhang, Yanhui[1];Li, Jian[2];Guo, Yuan[1]

机构：[1]Northwest Univ, Coll Chem & Mat Sci, Natl Demonstrat Ctr Expt Chem Educ, Key Lab Synthet & Nat Funct Mol Chem,Minist Educ, Xian 710127, Shaanxi, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Northwest Univ, Minist Educ, Key Lab Resource Biol & Biotechnol Western China, Xian 710069, Shaanxi, Peoples R China

年份：2019

卷号：283

起止页码：820

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000455854000099)】；

基金：This work was supported by the National Natural Science Foundation of China (Nos. 21472148 and 21072158), Open Funding Project of the State Key Laboratory of Bioreactor Engineering, Overseas Students Science and Technology Activities Project Merit Funding of Shaanxi Province (No. 20151190) and Academic Backbone of Northwest University Outstanding Youth Support Program.

语种：英文

外文关键词：Mitochondria-targeted; Fluorescent; Probe; Thiophenol; Oxidative stress; Damage; Live-cell; Imaging

摘要：Mitochondria as cellular powerhouses are the preferential targets affected by thiophenols, an important class of highly toxic environmental pollutants, and are linked to the production of pathogenic reactive oxygen species (ROS) induced by trace thiophenol residues. For real-time and accurate sensing, it is critically important to develop highly sensitive fluorescent probes for the specific detection of mitochondrial thiophenols. Herein, we report the first mitochondria-targeted fluorescent probe (ROAL) to image thiophenols in living cells. The development of ROAL was based on a novel red-emitting rhodol derivative (ROAP). ROAL proved to be highly selective to thiophenols among various analytes including aliphatic thiols, and renders an ultrasensitive off-on fluorescence response to thiophenols with a remarkable detection limit (8.1 nM). The probe efficiently stains mitochondria with a high Pearson's co-localization coefficient (0.95) using Mito Tracker Green FM as reference, thereby ensuring the specific detection of mitochondrial thiophenols in living HepG2 and HeLa cells. In particular, using this probe we for the first time proved that endogenous reactive oxygen species have the capacity to eliminate thiophenols in living cells, suggesting that thiophenols might induce cellular oxidative stress.