文献类型：期刊文献

英文题名：Interactions between bovine serum albumin and gemini surfactant alkanediyl-α, ω-bis(dimethyldodecyl-ammonium bromide)

作者：Pi, Yingying[1]; Shang, Yazhuo[1]; Peng, Changjun[1]; Liu, Honglai[1]; Hu, Ying[1]; Jiang, Jianwen[2]

机构：[1] Department of Chemistry, Lab. for Advanced Materials, East China University of Science and Technology, Shanghai 200237, China; [2] Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576, Singapore

年份：2006

卷号：83

期号：3

起止页码：243

外文期刊名：Biopolymers

收录：EI(收录号：20064110159568)

语种：英文

外文关键词：Complexation - Fluorescence - Hydrophobicity - Light scattering - Micelles

摘要：Interactions between bovine serum albumin (BSA) and cationic gemini surfactant alkanediyl-α,ω-bis(dimethyldodecyl-ammonium bromide) (12-n-12, n = 3, 4, 6) in aqueous solution have been investigated by measuring fluorescence, UV-vis transmittance, dynamic lighting scattering, and circular dichroism. Compared to a traditional surfactant dodecyltrimethylammonium bromide (DTAB), 12-n-12 interacts with BSA more strongly. With increasing concentration, 12-n-12 first binds specifically onto BSA leading to the unfolding and aggregation of BSA, and the decrease in α-helix content; and then forms micelle-like complexes along the unfolded BSA chains. A gemini surfactant with a longer spacer has a larger effect on BSA unfolding due to a stronger hydrophobic interaction. ? 2006 Wiley Periodicals, Inc.