文献类型：期刊文献

英文题名：An Integrated Control Strategy for the Fermentation of the Marine-Derived Fungus Aspergillus glaucus for the Production of Anti-cancer Polyketide

作者：Cai, Menghao[1];Zhou, Xiangshan[1];Lu, Jian[1];Fan, Weimin[1];Zhou, Jiushun[1];Niu, Chuanpeng[1];Kang, Li[1];Sun, Xueqian[1];Zhang, Yuanxing[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2012

卷号：14

期号：6

起止页码：665

外文期刊名：MARINE BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000310969000001)】；

基金：This work was supported by the National High Technology Research and Development Program of China (nos. 2010AA09Z402, 2011AA090702) and Shanghai Rising-Star Program (09QA1401600). We thank Prof. Qianqun Gu, Ocean University of China for supply of the strain and the aspergiolide A standard.

语种：英文

外文关键词：Aspergillus glaucus; Fermentation scale-up; Aspergiolide A; pH shift; Marine-derived fungus

摘要：An integrated control strategy of pH, shear stress, and dissolved oxygen tension (DOT) for fermentation scale-up of the marine-derived fungus Aspergillus glaucus HB 1-19 for the production of the anti-cancer compound aspergiolide A was studied. Keeping initial pH of 6.5 and shifting pH from 6.0 to 7.0 intermittently during the production phase greatly facilitated biosynthesis of aspergiolide A in shake flask cultures. Thus, a pH-shift strategy was proposed that shifting pH to 7.0 once it went lower than 6.0 by pulsed feeding NaOH solution during the production phase in bioreactor fermentation of A. glaucus HB 1-19. As a result, aspergiolide A production in a 30-L bioreactor was increased to 37.6 mg/L, which was 48.6% higher than that in 5-L bioreactor without pH shift. Fermentation scale-up was then performed in a 500-L bioreactor on the basis of an integrated criterion of near-same impeller tip velocity of early phase, DOT levels, and pH shift. The production of aspergiolide A was successfully obtained as 32.0 mg/L, which was well maintained during the process scale-up. This work offers useful information for process development of large-scale production of marine microbial metabolites.