文献类型：期刊文献

英文题名：STUDY OF VINYL SULFONYL REACTIVE DYE INTERMEDIATES IN THE COIMMOBILIZATION OF ENZYMES AND COENZYMES

作者：XU, J; ZHU, ZH; LOU, YX

机构：[1]E CHINA UNIV CHEM TECHNOL,FINE CHEM RES INST,130 MEILONG RD,SHANGHAI 200237,PEOPLES R CHINA

年份：1991

卷号：16

期号：1

起止页码：11

外文期刊名：DYES AND PIGMENTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:A1991FN29600002)】；

语种：英文

摘要：p-Aminobenzenesulfonylethyl-CL agarose was activated by diazotization, and with condensation with CS2 and glutaraldehyde to give the diazonium salt, isothiocyanate or aldehyde respectively. These were then used for the immobilization and coimmobilization of NAD, alcohol dehydrogenase and lactate dehydrogenase. The NAD immobilization ratio and activity recovery of immobilized enzymes were determined. The NAD regeneration ability of the site-to-site coimmobilized enzyme and coenzyme system was investigated.