文献类型：期刊文献

英文题名：Production and distribution of β-glucosidase in a mutant strain Trichoderma viride T 100-14

作者：Wang, Yong-Hong[1];Zhou, Jin[1];Chu, Ju[1];Qian, Jiang-Chao[1];Zhang, Si-Liang[1];Zhuang, Ying-Ping[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2009

卷号：26

期号：3-4

起止页码：150

外文期刊名：NEW BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000272267400007)】；

基金：This work was supported by the National Basic Research Program (973 Program, No. 2007CB714306), the National Special Fund for State Key Laboratory of Bioreactor Engineering (Grant No. 2060204) and East China University of Science and Technology.

语种：英文

摘要：The characterization of beta-glucosidase's production and distribution in a mutant strain Trichoderma viride T 100-14 at extracellular and intracellular levels were studied in this paper. Three experiment groups were done automatically with pH controlled at 4.8 during fermentation process, with 1 mg/ml 2-deoxy-D-glucose addition or without pH control and 2-deoXy-D-glucose addition (control). Activity assay and electron microscopic immunogold labeling experiments were performed at different culture periods (24, 48, 72, 96 and 120 hours). Under constant pH 4.8, high density of immunogold labeling particles, highest intracellular enzyme activity, total enzyme activity and specific activity were observed at 24 hours of fermentation. After 72 hours, the extracellular and total activities fluctuated little and the maximal activity in extracellular fraction was 2.7 times higher than control. By contrast, with 2-deoxy-D-glucose addition, the secreted and total beta-glucosidase activities achieved their maximum at 96 hours of fermentation, and the maximal secreted activity increased 2.05-fold than the control. Additionally, the secretion ratio (maximal secreted beta-glucosidase activity/maximal total activity) with pH control or 2-deoxy-D-glucose addition was elevated profoundly near to a level as the cellulase in fungi.