文献类型：期刊文献

英文题名：Differential Nanoscale Topography Dedicates Osteocyte-Manipulated Osteogenesis via Regulation of the TGF-β Signaling Pathway

作者：Cui, Jingyuan[1,2];Yang, Yaru[3];Chen, Peiru[4];Hang, Ruiqiang[5];Xiao, Yin[6];Liu, Xueting[1];Zhang, Lixin[1];Sun, Hui;Bai, Long[1,2,7]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Coll Mat Sci & Engn, Frontiers Sci Ctr Mat & Dynam Chem, Shanghai 200237, Peoples R China;[3]Jiaxing Univ, Coll Mat & Text Engn, Jiaxing 314001, Peoples R China;[4]Natl Ctr Prot Sci Beijing, Inst Life, Beijing Proteome Res Ctr, State Key Lab Prote, Beijing 102206, Peoples R China;[5]Taiyuan Univ Technol, Coll Mat Sci & Engn, Shanxi Key Lab Biomed Met Mat, Taiyuan 030024, Peoples R China;[6]Queensland Univ Technol, Australia China Ctr Tissue Engn & Regenerat Med, Brisbane, Qld 4000, Australia;[7]Shanghai Jiao Tong Univ, Dept Orthoped, Sch Med, Shanghai 200233, Peoples R China

年份：2022

卷号：23

期号：8

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000787510300001)】；

基金：This research was funded by the National Natural Science Foundation of China (No. 81901898) and the China Postdoctoral Science Foundation (No. 2019M661404, No. 2020T130192).

语种：英文

外文关键词：osteocyte; nanoscale; osteogenesis; TGF-beta; osseointegration

摘要：Osteocytes function as the master orchestrator of bone remodeling activity in the telophase of osseointegration. However, most contemporary studies focus on the manipulation of osteoblast and/or osteoclast functionality via implant surface engineering, which neglects the pivotal role of osteocytes in de novo bone formation. It is confirmative that osteocyte processes extend directly to the implant surface, but whether the surface physicochemical properties can affect the functionality of osteocytes and determine the fate of the osseointegration in the final remodeling stage remains to be determined. Titania nanotube arrays (NTAs) with distinct diameters were fabricated to investigate the relationship between the nanoscale topography and the functionality of osteocytes. In vitro results pinpointed that NTA with a diameter of 15 nm (NTA-15) significantly promote osteogenesis of osteocytes via the enhancement of spreading, proliferation, and mineralization. The osteocyte transcriptome of each group further revealed that the TGF-beta signaling pathway plays a pivotal role in osteocyte-mediated osteogenesis. The in vivo study definitely mirrored the aforementioned results, that NTA-15 significantly promotes bone formation around the implant. Consequently, nanoscaled topography-induced osteocyte functionality is important in late osseointegration. This suggests that surface designs targeting osteocytes may, therefore, be a potential approach to solving the aseptic loosening of the implant, and thus strengthen osseointegration.