文献类型：期刊文献

英文题名：Molecular networking assisted discovery and biosynthesis elucidation of the antimicrobial spiroketals epicospirocins

作者：Zhu, Guoliang[1];Hou, Chengjian[1];Yuan, Weize[1];Wang, Zhenzhen[1];Zhang, Jingyu[1];Jiang, Lan[1];Karthik, Loganathan[2];Li, Bixiao[1];Ren, Biao[3,4];Lv, Kangjie[1];Lu, Wanying[1];Cong, Zhanren[1];Dai, Huanqin[5];Hsiang, Tom[6];Zhang, Lixin[1];Liu, Xueting[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Salem Microbes Private Ltd, R&D Ctr, Salem, Tamilnadu, India;[3]Sichuan Univ, West China Hosp Stomatol, State Key Lab Oral Dis, Chengdu 610041, Peoples R China;[4]Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, Chengdu 610041, Peoples R China;[5]Chinese Acad Sci, Key Lab Pathogen Microbiol & Immunol, Inst Microbiol, Beijing 100101, Peoples R China;[6]Univ Guelph, Sch Envimnmental Sci, Guelph, ON N1G 2W1, Canada

年份：2020

卷号：56

期号：70

起止页码：10171

外文期刊名：CHEMICAL COMMUNICATIONS

收录：;EI(收录号：20203809187213);WOS:【SCI-EXPANDED(收录号:WOS:000564463200011)】；

基金：We gratefully acknowledge the financial support from the National Key Research and Development Program of China (2019YFA0906200), the National Natural Science Foundation of China (21977029, 81903529, 21877038, 21907031, 31720103901, and 81573341), the Open Project Funding of the State Key Laboratory of Bioreactor Engineering, the 111 Project (B18022), and Shanghai Science and Technology Commission (18JC1411900). The authors acknowledge the Australian Research Council (ARC) for support (LE140100119, LE20100170).

语种：英文

外文关键词：Biochemistry - Chirality

摘要：Two pairs of dibenzospiroketal racemates, (+/-)-epicospirocin A (1a/1b) and (+/-)-1-epi-epicospirocin A (2a/2b), and two (+)-enantiomers of aspermicrones,ent-aspermicrone B (3b) andent-aspermicrone C (4b), together with two hemiacetal epimeric mixtures, epicospirocin B/1-epi-epicospirocin B (5/6) and epicospirocin C/1-epi-epicospirocin C (7/8), were investigated from the phytopathogenic fungusEpicoccum nigrum09116viaMS/MS molecular networking guided isolation and chiral separation for the first time. A plausible epicospirocin biosynthetic pathway was elucidated throughin silicogene function annotation together with knock-out experiments. This is the first report that has applied MS/MS molecular networking to identify intermediates correlated with a biosynthetic pathway.