文献类型：期刊文献

英文题名：Biocompatible graphene nanosheets grafted with poly(2-hydroxyethyl methacrylate) brushes via surface-initiated ARGET ATRP

作者：Sha, Jin[1,2];Gao, Yuan[1];Wu, Tong[1,2];Chen, Xin[1,2];Cordie, Travis[2];Zhao, Haili[1];Xie, Linsheng[1];Ma, Yulu[1];Turng, Lih-sheng[2]

机构：[1]E China Univ Sci & Technol, Minist Educ, Engn Ctr Efficient Green Proc Equipment & Energy, Shanghai 200237, Peoples R China;[2]Univ Wisconsin, Wisconsin Inst Discovery, Madison, WI 53715 USA

年份：2016

卷号：6

期号：42

起止页码：35641

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20161902367650);WOS:【SCI-EXPANDED(收录号:WOS:000374349600058)】；

基金：The authors sincerely acknowledge the support of Fundamental Research Funds for the Central Universities (22A201514030), China Postdoctoral Science Foundation (2015M571504), National Natural Science Foundation of China (5150306, 551273065) and the Wisconsin Institute for Discovery in University of Wisconsin-Madison.

语种：英文

外文关键词：Biocompatibility - Dendrimers - Medical applications - Nanosheets - Atom transfer radical polymerization - Grafting (chemical) - Nanostructured materials - Platelets - Tin oxides - Assays - Copper - Endothelial cells - Cell culture - Catalysts - Proteins

摘要：Using robust chemistry to graft polymer brushes on graphene nanosheets would promote the development of graphene nanomaterials as a versatile platform for biomedical applications. Based on surface-initiated activators regenerated by the electron transfer atom transfer radical polymerization (ARGET ATRP) technique, the study developed a protocol to prepare well-defined poly(2-hydroxyethyl methacrylate) (HEMA) brushes on chemically reduced graphene oxide surfaces. ATR-FTIR, XPS and TEM characterizations demonstrate tin(II) 2-ethylhexanoate to be an efficient reducing agent that provides controlled polymerization with a significant decreased Cu catalyst usage (down to about 20 ppm), and prevents trace amounts of elemental Cu residue on the graphene surface. Fetal bovine serum protein absorption assay reveals the effect of brush backbone structure change to tune the interfacial interaction between graphene nanosheets and proteins. Further, NIH-3T3 fibroblast cell and human umbilical vein endothelial cell viability assays indicate that the obtained graphene nanosheets meet the biocompatibility requirements to support fibroblast cells, even human cells, attach and proliferate. The approach and the graphene-polymer brush hybrid developed in this work should open new opportunities for a broader range of biomedical applications of carbon nanomaterials.