文献类型：期刊文献

中文题名：大肠杆菌耦合乙酸分离的过滤培养

英文题名：Cultivation Integrated with Acetate Acid Filtration on Escherichia coli

作者：杜鹏[1];叶勤[1];俞俊棠[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2000

卷号：16

期号：4

起止页码：528

中文期刊名：生物工程学报

外文期刊名：Chinese Journal of Biotechnology

收录：CSTPCD;;Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；PubMed;

基金：国家自然科学基金重点资助项目!( 2 92 3 614 0 )

语种：中文

中文关键词：发酵分离耦合;乙酸;干扰素;大肠杆菌;E.coli

外文关键词：Integrated process, acetate acid,interferon, Escherichia coli

摘要：A fed batch fermentation integrated with filtration process was proceeded in a synthetic medium with the use of a hollow fibre membrane filter.The activity of α A interferon reached 1.4×10 9u/L,which was increased 320% over that of a control process.The integrated process was then proceeded in the synthetic medium supplemented with yeast extract during the earlier stage.The addition of yeast extract not only reduced the accumulation of acetate,but also promoted the production of α A interferon.The maximum activity achieved 1.9×10 9 u/L during the fermentation,which was increased 480% over that of a normal process.
A fed batch fermentation integrated with filtration process was proceeded in a synthetic medium with the use of a hollow fibre membrane filter.The activity of α A interferon reached 1.4×10 9u/L,which was increased 320% over that of a control process.The integrated process was then proceeded in the synthetic medium supplemented with yeast extract during the earlier stage.The addition of yeast extract not only reduced the accumulation of acetate,but also promoted the production of α A interferon.The maximum activity achieved 1.9×10 9 u/L during the fermentation,which was increased 480% over that of a normal process.