文献类型：期刊文献

中文题名：苯高效降解菌群富集及其降解机理研究

英文题名：The enrichment of benzene high-efficiency degradation bacteria community and its degradation mechanism

作者：张磊[1];罗启仕[1,2];陈欣[1];沈佳敏[1];王震[1];张峰[3];吕树光[1];林匡飞[1];崔长征[1]

机构：[1]华东理工大学资源与环境工程学院,国家环境保护化工过程环境风险评价与控制重点实验室,上海市环境保护化学污染物环境标准与风险管理重点实验室,上海200237;[2]永清环保股份有限公司上海分公司,上海200051;[3]上海格林曼环境技术有限公司,上海200001

年份：2021

卷号：41

期号：9

起止页码：3770

中文期刊名：环境科学学报

外文期刊名：Acta Scientiae Circumstantiae

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家重点研发计划重点专项(No.2018YFC1803300)。

语种：中文

中文关键词：原位修复;苯系物(BTEX);生物降解;代谢途径;群落结构

外文关键词：in-situ remediation;BTEX;biodegradation;metabolic pathway;community structure

摘要：微生物绿色、低耗、原位修复去除污染土壤与地下水中可降解污染物日益受到关注.本研究从江苏某农药厂苯系物污染土壤中以苯为唯一碳源富集获得苯降解菌群.在28℃、pH=8.0、添加100 mg·L^(-1)酵母粉条件下,该菌群在6 h内完全降解100 mg·L^(-1)的苯,利用Q-TOF解析出苯酚和邻苯二酚等主要代谢产物,并通过PCR扩增出苯酚单加氧酶基因(GenBank登录号:MW388722).该菌群30 h内可完全降解30 mg·L^(-1)的苯、甲苯和乙苯混合物,高通量测序结果显示该菌群主要含有Cupriavidus、Arthrobacter、Dyella、Corynebacterium、Microbacterium等菌属,其中Cupriavidus为优势菌,菌群群落结构稳定且可高效降解苯,具有潜在的应用前景.
The removal of degradable pollutants in contaminated soil and groundwater by the green, low-consumption, in-situ microbial remediation is attracting increasing attention. In this study, a benzene-degrading bacteria community was isolated from the contaminated soil polluted by benzene series in a pesticide factory in Jiangsu Province and enriched with benzene as the only carbon source. Under the temperature of 28 ℃, pH value of 8.0 and the addition of 100 mg·L^(-1) yeast powder, this bacterial community could completely degrade 100 mg·L^(-1) of benzene within 6 hours. The main metabolites such as phenol and catechol were analyzed using Q-TOF, and the phenol monooxygenase gene was amplified using PCR amplification and sequenced(GenBank accession number: MW388722). What′s more, this bacterial community could completely degrade the mixture of 30 mg·L^(-1) of benzene, toluene and ethylbenzene within 30 hours. High-throughput sequencing results showed that this bacterial community mainly contained Cupriavidus, Arthrobacter, Dyella, Corynebacterium, Microbacterium bacterial species, with Cupriavidus being the dominant bacteria. This bacteria community has stable structure, can rapidly degrades benzene, has good application prospects.