文献类型：期刊文献

中文题名：L-谷氨酸氧化酶高产菌株的快速筛选及诱变育种

英文题名：Breeding of L-glutamate Oxidase Producing Strains with Mutagenesis and a Rapid Screening Method

作者：李青山[1];李友荣[1];闵简书[1]

机构：[1]华东化工学院生化工程研究所

年份：1993

卷号：19

期号：2

起止页码：148

中文期刊名：华东化工学院学报

外文期刊名：Huadong Huagong Xueyuan Xuebao/Journal of East China Institute of Chemical Technology

收录：国家哲学社会科学学术期刊数据库;EI(收录号：1994031239838);Scopus;CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：谷氨酸;育种;筛选;氧化酶

外文关键词：glutamic acid;mutation breeding;screening;oxidase;plate color development;L-glutamate

摘要：用谷氨酸氧化酶测试液浸湿白卷纸置于菌落上,根据白卷纸上显示红色快慢、深浅,就能大致测得该菌落菌株产酶能力高低。该法可靠性大、工作量小、操作简便,显示结果快。利用该法,配合亚硝酸诱变,在短暂三个月内,将L-谷氨酸氧化酶产生菌株产酶能力提高了约25倍。
When two pieces of white tissue moistened with L-glutamate oxidase(GO) assayed solution were placed on the mature colonies, red spots developed. The speed and the extent of color development could be used to estimate the productivity of the strain tested. By using curent screening method along with HNO_2 mutagenesis, the productivity of GO producing strain P-26 is increased about 25 times within three manthes.