文献类型：期刊文献

英文题名：Copper-mediated on-off control of gene expression in filamentous fungus Trichoderma reesei

作者：Wang, Wei[1];Chen, Yumeng[1];Wei, Dong-Zhi[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, New World Inst Biotechnol, Shanghai 200237, Peoples R China

年份：2017

卷号：143

起止页码：63

外文期刊名：JOURNAL OF MICROBIOLOGICAL METHODS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000416880600012)】；

基金：This research was supported by the National Natural Science Foundation of China (No. C010302-31500066) and the Fundamental Research Funds for the Central Universities (No. 222201714053).

语种：英文

外文关键词：Trichoderma reesei; Filamentous fungus; Copper responsive promoter; On-off control; Unmarked genetic modification; Marker rescue; Gene expression; Cre/loxP

摘要：We developed a copper-mediated gene expression on-off control system and an unmarked genetic modification strategy based on the modified copper responsive promoter Ptculc from the filamentous fungus Trichoderma reesei. The molecular tool for the gene expression on-off control can be used to manipulate many biological processes in T. reesei.