文献类型：期刊文献

英文题名：Layer-by-layer self-assembly of minocycline-loaded chitosan/alginate multilayer on titanium substrates to inhibit biofilm formation

作者：Lv, Hongbin[1];Chen, Zhen[1];Yang, Xiaoping[2];Cen, Lian[3,4];Zhang, Xu[1];Gao, Ping[1]

机构：[1]Tianjin Med Univ, Sch Stomatol, Tianjin 300070, Peoples R China;[2]Beijing Univ Chem Technol, Key Lab Beijing City Preparat & Proc Novel Polyme, Beijing 100029, Peoples R China;[3]Natl Tissue Engn Ctr China, Shanghai 200241, Peoples R China;[4]E China Univ Sci & Technol, Sch Chem Engn, Shanghai 200237, Peoples R China

年份：2014

卷号：42

期号：11

起止页码：1464

外文期刊名：JOURNAL OF DENTISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000344618000013)】；

基金：This work was jointly supported by National Science and Technology Support Project Foundation (Grant No: 2012BAI07B00), National "973" Project Foundation (Grant No: 2010CB944804), National Natural Science Foundation of China (Grant No: 81200817) and Tianjin Research Program of Application Foundation and Advanced Technology (Youth Research Program) (Grant No: 12JCQNJC09200).

语种：英文

外文关键词：Titanium implant; Biofilm; Layer-by-layer self-assembly; Antibacterial coating; Chitosan; Alginate

摘要：Objectives: Bacteria adhesion and subsequent biofilm formation are primary causes of implant associated infection. The biofilm makes the bacteria highly resistant to the host defense and antimicrobial treatment. Antibacterial coatings on the surface of titanium implant can prevent biofilm formation effectively, but it is still a challenge to accomplish relatively long lasting antibacterial effects before wound healing or formation of biological seal. The purpose of our work was to construct antibacterial multilayer coatings loaded with minocycline on surface of Ti substrates using chitosan and alginate based on layer-by-layer (LbL) self-assembly technique. Methods: In this study, the surfaces of Ti substrates were first hydroxylated and then treated with 3-aminopropyltriethoxysilane (ATPES) to obtain amino-functionalized Ti substrates. Next, the precursor layer of chitosan was covalently conjugated to amino-functionalized Ti substrates. The following alternately coating alginate loaded with minocycline and chitosan onto the precursor layer of chitosan was carried out via LbL self-assembly technique to construct the multilayer coatings on Ti substrates. Results: The multilayer coatings loaded more minocycline and improved sustainability of minocycline release to kill planktonic and adherent bacteria. Moreover, surface charge and hydrophilicity of the coatings and antibacterial ability of chitosan itself also played roles in the antibacterial performance, which can keep the antibacterial ability of the multilayer coatings after minocycline release ceases. Conclusions: In conclusion, LbL self-assembly method provides a promising strategy to fabricate long-term antibacterial surfaces, which is especially effective in preventing implant associated infections in the early stage. Clinical significance: Loading minocycline on the surface of implants based on LbL self-assembly strategy can endow implants with sustained antibacterial property. This can inhabit the immediate colonization of bacteria onto the surface of implants in the process of dental implant surgery, and thereby prevents and reduces the occurrence of periimplantitis. (C) 2014 Elsevier Ltd. All rights reserved.