文献类型：期刊文献

英文题名：Structure elucidation and immunological activity of a novel exopolysaccharide from Paenibacillus bovis sp. nov BD3526

作者：Zhu, Shiming[1];Han, Jin[2];Yan, Zhichao[1];Wu, Yuze[1];Zhang, Wenqing[1];Xia, Wei[1];Feng, Huafeng[3]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Bright Dairy & Food Co Ltd, Dairy Res Inst, State Key Lab Dairy Biotechnol, Shanghai Engn Res Ctr Dairy Biotechnol, Shanghai 200436, Peoples R China;[3]Shanghai Business Sch, Dept Food Sci, Shanghai 201400, Peoples R China

年份：2022

卷号：282

外文期刊名：CARBOHYDRATE POLYMERS

收录：;EI(收录号：20220311465848);WOS:【SCI-EXPANDED(收录号:WOS:000760993500002)】；

基金：This work was supported by the Scientific Fund (19DZ2281400) of Shanghai Engineering Research Center of Dairy Biotechnology.

语种：英文

外文关键词：Paenibacillus bovis sp; nov BD3526; Exopolysaccharide; Purification; Structure elucidation; Immunological activity

摘要：A novel exopolysaccharide named BD0.4 was purified from the fermentation broth of Paenibacillus bovis sp. nov BD3526 in wheat bran medium via anion exchange column chromatography. Its fine structure was identified by a variety of physical and chemical methods. BD0.4, with the weight average molecular weight of 376 kDa, consisted of glucuronic acid, glucose and fucose in a molar ratio of 1.58:1:1.66. The backbone included 1,3 linked Fuc, 1,3,4-linked Fuc, 1,3-linked Glc and 1,4-linked GlcA residues, with the branching point located at the O4 position of 1,3,4-linked Fuc residues, and the branched chain composed of terminal GlcA residues. BD0.4 could improve the phagocytic ability of macrophages and significantly stimulate the secretion of NO, TNF-alpha, IL1 beta and IL-6 from RAW264.7 cells in a dose-dependent manner. BD0.4 could promote the expression of NF-kB and cause nuclear translocation of NF-kappa B p65, indicating that BD0.4 probably exerted immune activity through the NF-kappa B signaling pathway.