文献类型：期刊文献

英文题名：Mechanistic insight for improving butenyl-spinosyn production through combined ARTP/UV mutagenesis and ribosome engineering in Saccharopolyspora pogona

作者：Zhao, Xueli[1]; Hussain, Muhammad Hammad[1]; Mohsin, Ali[1]; Liu, Zebo[1]; Xu, Zhixian[1]; Li, Zhanxia[2]; Guo, Weiqun[3]; Guo, Meijin[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China; [2] Department of Respiratory Medicine, Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China; [3] Academy of National Food and Strategic Reserves Administration, Beijing, China

年份：2023

卷号：11

外文期刊名：Frontiers in Bioengineering and Biotechnology

收录：EI(收录号：20240515480207)

语种：英文

外文关键词：Biochemistry - Macromolecules - Mutagenesis

摘要：Butenyl-spinosyn is a highly effective, wide-spectrum and environmentally-friendly biological insecticide produced by Saccharopolyspora pogona. However, its scale-up is impeded due to its lower titer in wild-type strains. In this work, ARTP/UV mutagenesis and ribosome engineering were employed to enhance the butenyl-spinosyn production, and a stable mutant Saccharopolyspora pogona aG6 with high butenyl-spinosyn yield was successfully obtained. For the first time, the fermentation results in the 5L bioreactor demonstrated that the butenyl-spinosyn produced by mutant Saccharopolyspora pogona aG6 reached the maximum value of 130mg/L, almost 4-fold increase over the wild-type strain WT. Furthermore, comparative genomic, transcriptome and target metabolomic analysis revealed that the accumulation of butenyl-spinosyn was promoted by alterations in ribosomal proteins, branched-chain amino acid degradation and oxidative phosphorylation. Conclusively, the proposed model of ribosome engineering combined with ARTP/UV showed the improved biosynthesis regulation of butenyl-spinosyn in S. pogona. Copyright ? 2024 Zhao, Hussain, Mohsin, Liu, Xu, Li, Guo and Guo.