文献类型：期刊文献

中文题名：抗生素Bagremycin生产菌Streptomyces sp. Tü4128接合转移系统的建立

英文题名：Development of an Intergeneric Conjugal Transfer System for Streptomyces sp. Tü 4128,a Producer of Bagremycins

作者：朱云霞[1];廖思懿[1];叶江[1];张惠展[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2012

卷号：38

期号：2

起止页码：160

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：attB位点;噬菌体φC31;bagremycin;属间接合;Streptomyces;sp.;Tü4128

外文关键词：attB site; bacteriophage φC31; bagremycin; intergeneric conjugation; Streptomyces sp.Tü 4128

摘要：基于噬菌体φC31 att/int系统,采用属间接合将来自大肠杆菌的DNA有效地导入菌株Tü4128的孢子中。通过条件优化,当融合温度为37℃时,在含80mmol/L MgCl2的ISP4培养基上使每个受体细胞的接合频率显著提高到7.3×10-3。Southern blot分析显示在受体菌染色体上存在单一的染色体附着位点(attB位点)。整合位点的DNA序列测序显示为保守。采用优化的方法建立了大肠杆菌和菌株Tü4128间高效的属间接合系统。
DNA from Escherichia coli was successfully introduced into spores of strain Tü 4128 by intergeneric conjugation through bacteriophage φC31 att/int system.The transconjugation frequency on ISP4 medium with 80 mmol/L MgCl2 was significantly improved up to 7.3×10-3 per recipient cell under optimized mating temperature of 37 ℃.Southern blot analysis revealed a single chromosomal attachment site on the chromosome of the recipient strain.The DNA sequence of the integration site was determined and shown to be conserved.An efficient intergeneric conjugation between Escherichia coli and strain Tü 4128 was successfully achieved by condition optimization.