文献类型：期刊文献

英文题名：Nanoconfined Electrokinetic Chromatography (NEC): Gradient Separation and Sensing of Short DNA Fragments at the Single-Molecule Level

作者：Lv, Jian[1];Wu, Xue[1];Wu, Mansha[1];Wang, Xiaoyuan[1];Gong, Lijuan[1];Li, Dawei[1];Qian, Ruocan[1]

机构：[1]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China

年份：2024

卷号：96

期号：14

起止页码：5702

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20241415839757);WOS:【SCI-EXPANDED(收录号:WOS:001193653800001)】；

基金：This research was supported by the National Natural Science Foundation of China (21974046, 21977031, 22176058), the China Postdoctoral Science Foundation (2021M701195), Shanghai Science and Technology Committee (19520744000, 22ZR1416800, 23ZR1416100), and the Program of Introducing Talents of Discipline to Universities (B16017). The authors thank the Research Center of Analysis and Test of East China University of Science and Technology for the help on characterization.

语种：英文

外文关键词：DNA - Electrodynamics - Gene transfer - Liquid chromatography

摘要：Glass nanopipets have been demonstrated to be a powerful tool for the sensing and discrimination of biomolecules, such as DNA strands with different lengths or configurations. Despite progress made in nanopipet-based sensors, it remains challenging to develop effective strategies that separate and sense in one operation. In this study, we demonstrate an agarose gel-filled nanopipet that enables hyphenated length-dependent separation and electrochemical sensing of short DNA fragments based on the electrokinetic flow of DNA molecules in the nanoconfined channel at the tip of the nanopipet. This nanoconfined electrokinetic chromatography (NEC) method is used to distinguish the mixture of DNA strands without labels, and the ionic current signals measured in real time show that the mixed DNA strands pass through the tip hole in order according to the molecular weight. With NEC, gradient separation and electrochemical measurement of biomolecules can be achieved simultaneously at the single-molecule level, which is further applied for programmable gene delivery into single living cells. Overall, NEC provides a multipurpose platform integrating separation, sensing, single-cell delivery, and manipulation, which may bring new insights into advanced bioapplication.