文献类型：期刊文献

英文题名：Living Seed Materials Made by Metal-Organic Framework-Encapsulated Bacillus Subtilis Spore

作者：Liu, Yi[1,2,3];Wu, Mengyao[1];Kang, Anqi[1];Zhang, Xiehaoran[1];Xie, Ronghui[4];Huang, Yuanpei[5];Huang, Jiaofang[5];Chou, Lien-Yang[1];Chao, Zhong[2,3]

机构：[1]ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201203, Peoples R China;[2]Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Chinese Acad Sci, Ctr Mat Synthet Biol, Shenzhen 518055, Peoples R China;[3]Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Key Lab Quantitat Synthet Biol, Shenzhen 518055, Peoples R China;[4]Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Chinese Acad Sci, Ctr Cell & Gene Circuit Design, Shenzhen 518055, Peoples R China;[5]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200207, Peoples R China

年份：2024

卷号：34

期号：11

外文期刊名：ADVANCED FUNCTIONAL MATERIALS

收录：;EI(收录号：20234915180813);WOS:【SCI-EXPANDED(收录号:WOS:001114948000001)】；

基金：Y.L. and M.Y.W. contributed equally to this work. This work was sponsored by the National Key R&D Program of China (Grant No. 2020YFA0908100), the National Natural Science Foundation of China (Grant No. 32125023), start-up funding from ShanghaiTech University,the National Natural Science Foundation of China (key program no. U1932204), Shenzhen Science and Technology Program (ZDSYS20220606100606013), and the Natural Science Foundation of Shanghai (Grant No. 22ZR1416000). The authors thank the support from the Analytical Instrumentation Center (Contract No. SPST-AIC10112914), School of Physical Science and Technology, ShanghaiTech University. SEM and TEM experiments were supported by the Center for High-resolution Electron Microscopy (CyEM) at ShanghaiTech University.

语种：英文

外文关键词：bacillus subtilis spores; biohybrid materials; biologically inspired materials; living materials; metal-organic frameworks

摘要：Genetically modified microorganisms (GMMs) possess significant potential for a wide range of applications; however, their execution in real-world scenarios, particularly in environments characterized by scarce nutrients and unpredictable conditions, proves daunting. Drawing inspiration from the adaptability of natural seeds, living seed materials that combine a core-shell structure ZIF-8 and genetically engineered spores are introduced. By genetically modifying the Bacillus subtilis strain to express distinct fluorescent proteins, the visualization of spores within the hollow sphere is facilitated, allowing for differentiation between spore and bacteria states. Importantly, the ZIF-8 shell exhibits responsiveness to diverse nutrient levels, providing enhanced environmental resistance in comparison to bare spores upon exposure to hypochlorous acid, UV-C radiation, and even vacuum conditions. Moreover, the potential of living seed materials for living sensors of small molecules is showcased by elucidating that spores can be resuscitated into functional bacteria after release from ZIF-8 material. This research establishes a novel approach for the general fabrication of Metal-Organic Frameworks (MOF)-based living spore hybrid materials, offering environmentally controlled release of living materials across a multitude of applications.