文献类型：期刊文献

英文题名：Hematopoietic reconstitution of CD34⁺ cells grown in static and stirred culture systems in NOD/SCID mice

作者：Yang, Shi; Cai, Haibo; Jin, Huili; Tan, Wen-Song

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2008

卷号：30

期号：1

起止页码：61

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000251307500009)】；

语种：英文

外文关键词：CD34(+) cells; ex vivo expansion; NOD; SCID mice; static culture; stirred culture

摘要：The hematopoietic reconstitution of cord blood (CB) CD34(+) cells grown in static and stirred system was studied. Static cultures were better than stirred cultures for cell expansion. Engraftment of stirred-culture hematopoietic stem cells (HSCs) was higher than static-culture HSCs. Stirred-culture HSCs had better multilineage reconstitution ability and colony-forming ability than static-culture HSCs. Static cultures thus favor the expansion of HSCs and stirred cultures are more effective in preserving functional HSCs.