文献类型：期刊文献

中文题名：毛细管电泳同步分离检测“瘦肉精”及其替代物

英文题名：Separation and Determination of Clenbuterol and Its Substitutes Simultaneously by Capillary Electrophoresis

作者：高杨菲[1];许学书[1]

机构：[1]华东理工大学生物器反应工程国家重点试验室,上海200237

年份：2008

卷号：29

期号：3

起止页码：71

中文期刊名：食品工业

外文期刊名：The Food Industry

收录：北大核心:【北大核心2004】；

语种：中文

中文关键词：毛细管电泳;克伦特罗;沙丁胺醇;特布他林;莱克多巴胺;β2-肾上腺素受体激动剂

外文关键词：capillary electrophoresis; clenbuterol; salbutamol; terbutaline; ractopamine; β2-adrenoceptor （AR） agonists

摘要：建立用毛细管电泳法同步分离检测肉制品中"瘦肉精"及其替代物的方法。该方法前处理简单,整个分离在12min内完成。以90mmol/L的柠檬酸三钠(pH5.6)为电泳缓冲液,8kV分离电压,200nm检测波长,在25℃下可使克伦特罗,沙丁胺醇,特布他林和莱克多巴胺达到分离,分离度分别为1.90,2.73和2.17,回收率为92.4%～102.0%,RSD<3.2%。
A simple and rapid method of capillary electrophoresis for separation and confirmation mixture simultaneously of four β2-adrenoceptor （AR） agonist, such as ractopamine （RAC）,clenbuterol（CLB）, salbuterol （ SAL ） and terbutaline （ TER ） was established. The effects of buffer concentration, pH, additive, voltage and temperature on separation were investigated. Optimum conditions have been achieved at 25℃, 90 mmol/L trisodium citrate buffer, pH5.6, at 8 kV, with direct detection at 200 nm. Under the optimum conditions, resolutions were 1.90, 2.73, and 2,17 for CLB, SAL, TER and RAC, respectively. Recovery were from 92.4% to102.0%, RSD〈3.2%.