文献类型：期刊文献

英文题名：The Safety Evaluation of Salvianolic Acid B and Ginsenoside Rg1 Combination on Mice

作者：Zhao, Qun[1,2];Yang, Min[1];Deng, Yanping[1];Yu, Haitao[1];Wang, Linlin[1];Teng, Fukang[1,2];Cho, Kenka[3];Ma, Hongmei[4];Wu, Peng[1,2];Li, Xue[1];Wu, Wanying[1];Liu, Xuan[1];Xu, Feng[2];Jiang, Baohong[1];Guo, De-An[1]

机构：[1]Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;[2]Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China;[3]Takarazuka Univ Med & Hlth Care, Takarazuka, Hyogo 6660162, Japan;[4]E China Univ Sci & Technol, Shanghai 200237, Peoples R China

年份：2015

卷号：16

期号：12

起止页码：29345

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000367535600087)】；

基金：This work was supported by the National Science & Technology Major Project for "Key New Drug Creation and Manufacturing Program" (2013ZX09103002-024), the National Natural Science Foundation of China Grants (81573646) and the Shanghai Science and Technology Development Foundation (14401900900). This work was also partially supported by the Croucher Foundation (CAS14201).

语种：英文

外文关键词：safety; salvianolic acid B; ginsenoside Rg1; acute toxicity; repeated toxicity

摘要：Our previous study indicated that the combination of salvianolic acid B (SalB) and ginsenoside Rg1 (Rg1), the main components of Salvia miltiorrhizae and Panax notoginseng, improves myocardium structure and ventricular function in rats with ischemia/reperfusion injury. The present study aimed to determine the safety of the combined SalB and Rg1 (SalB-Rg1) in mice. The safety of SalB-Rg1 was evaluated through acute toxicity and repeated-dose toxicity. In the acute toxicity study, the up and down procedure was carried out firstly, and then, the Bliss method was applied. In the toxicity study for seven-day repeated treatment of SalB-Rg1, forty Kunming mice were randomly divided into four groups. The intravenous median lethal dose (LD50) of the SalB-Rg1 combination was 1747 mg/kg using the Bliss method. For both the acute toxicity study and the seven-day repeated toxicity study, SalB-Rg1 did not induce significant abnormality on brain, heart, kidney, liver and lung structure at any dose based on H&E stain. There were no significant changes related to the SalB-Rg1 toxicity detected on biochemical parameters for two kinds of toxicity studies. The LD50 in mice was 1747 mg/kg, which was more than one hundred times higher than the effective dose. Both studies of acute toxicity and seven-day repeated dose toxicity indicated the safety of the SalB-Rg1 combination.