文献类型：期刊文献

英文题名：Microfluidic preparation of PLGA microspheres as cell carriers with sustainable Rapa release

作者：Zhu, Chengcheng[1];Yang, Haibo[1];Shen, Liang[1];Zheng, Zhuoyuan[1];Zhao, Shicheng[1];Li, Qingguo[2];Yu, Fengbin[3];Cen, Lian[1]

机构：[1]East China Univ Sci & Technol, Sch Chem Engn, Shanghai Key Lab Multiphase Mat Chem Engn, State Key Lab Chem Engn,Dept Prod Engn, Shanghai, Peoples R China;[2]Guangzhou Univ Chinese Med, Sch Pharmaceut Sci, Guangzhou, Guangdong, Peoples R China;[3]98 Hosp PLA, Dept Orthopaed Surg, Huzhou, Peoples R China

年份：2019

卷号：30

期号：9

起止页码：737

外文期刊名：JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION

收录：;EI(收录号：20191706827716);WOS:【SCI-EXPANDED(收录号:WOS:000465917100001)】；

基金：This study is financially supported by National Nature Science Foundation (81471855) and 2017GY38, and "Open Funding Project of the State Key Laboratory of Bioreactor Engineering".

语种：英文

外文关键词：PLGA microsphere; rapamycin; drug release; cell carrier; cell therapy

摘要：The current study, inspired by the immunosuppressive property of rapamycin (Rapa) and the benefit of microspheres both as drug delivery system and cell carriers, was designed to develop an efficient Rapa delivery system with tunable controllability to facilitate its local administration. A capillary-based two-phase microfluidic device was designed to prepare monodisperse poly(lactide-co-glycolide) (PLGA) microspheres to load Rapa (PLGA-Rapa-M). The physical and chemical properties of PLGA-Rapa-M were characterized, and the Rapa loading capacity and release profile were explored. Chondrocytes were chosen as a cell model to evaluate the adhesion and proliferation on these microspheres. Controllability over the microsphere properties was illustrated. The PLGA-Rapa-M is averagely 63.91 mu m in size with a narrow size distribution and a CV of 2.44%. The encapsulation efficiency of Rapa within microspheres via the current microfluidics was around 98%, and Rapa loading could be easily varied with a maximum value of similar to 20%. The PLGA-Rapa-M has a sustained Rapa release duration of similar to 3 months. These microspheres could not only successfully be used for Rapa sustained release but also as cell carriers for cell therapy since they can support the attachment/proliferation of chondrocytes. Hence, improved therapeutic index could be expected by using the current developed Rapa-release system. [GRAPHICS] .