文献类型：期刊文献

英文题名：Multicomponent mesofluidic system for the detection of veterinary drug residues based on competitive immunoassay

作者：Hu, Lei;Zuo, Peng;Ye, Bang-Ce[1]

机构：[1]E China Univ Sci & Technol, Lab Biosyst, Shanghai 200237, Peoples R China; E China Univ Sci & Technol, Microanal Lab, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2010

卷号：405

期号：1

起止页码：89

外文期刊名：ANALYTICAL BIOCHEMISTRY

收录：;EI(收录号：20201908623166);WOS:【SCI-EXPANDED(收录号:WOS:000280890600011)】；

基金：The authors thank Prof. Alastair Smith and Ed Quinn of the University of Leeds for many helpful suggestions. This work was financially supported by China NSF (20627005), the Shanghai Project (07dz19508, 09JC1404100), the Program for New Century Excellent Talents (NCET-07-0287), Shanghai Shuguang Program (06SG32), the National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204), and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Mesofluidic; Drug residues; Immunoassay; Multiplexed analysis

摘要：An automated multicomponent mesofluidic system (MCMS) based on biorecognitions carried out on meso-scale glass beads in polydimethylsiloxane (PDMS) channels was developed. The constructed MCMS consisted of five modules: a bead introduction module, a bioreaction module, a solution handling module, a liquid driving module, and a signal collection module. The integration of these modules enables the assay to be automated and reduces it to a one-step protocol. The MCMS has successfully been applied toward the detection of veterinary drug residues in animal-derived foods. The drug antigen-coated beads (phi 250 mu m) were arrayed in the PDMS channels (phi 300 mu m). The competitive immunoassay was then carried out on the surface of the glass beads. After washing, the Cy3-labeled secondary antibody was introduced to probe the antigen-antibody complex anchored to the beads. The fluorescence intensity of each bead was measured and used to determine the residual drug concentration. The MCMS is highly sensitive, with its detection limits ranging from 0.02 (salbutamol) to 3.5 mu g/L (sulfamethazine), and has a short assay time of 45 min or less. The experimental results demonstrate that the MCMS proves to be an economic, efficient, and sensitive platform for multicomponent detection of compound residues for contamination in foods or the environment. (C) 2010 Elsevier Inc. All rights reserved.