文献类型：会议论文

英文题名：A simple unstructured model-based control for efficient expression of recombinant porcine insulin precursor by Pichia pastoris

作者：Hang, Hai-feng[1];Chen, Wen[1];Guo, Mei-jin[1,2];Chu, Ju[1];Zhuang, Ying-ping[1];Zhang, Siliang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Jiangxi Agr Univ, Dept Biotechnol, Nanchang 330045, Peoples R China

会议论文集：13th YABEC Symposium 2007

会议日期：OCT 20-22, 2007

会议地点：Seoul, SOUTH KOREA

语种：英文

外文关键词：Pichia pastoris; Porcine Insulin Precursor; Monod Modeling; Methanol Feeding Strategy

摘要：Based on the fact that Pichia cell growth follows a Monod equation under the condition of methanol concentration limitation, a kinetics model of recombinant methylotrophic yeast Pichia pastoris expressing porcine insulin precursor (PIP) was developed in the quasi-steady state in the induction phase. The model revealed that the relationship between specific growth rate (mu) and substrate methanol concentration was in accord with the Monod equation. The fermentation kinetic parameters maximum specific growth rate (mu(max)), saturation constant (K(s)) and maintenance coefficient (M) were estimated to be 0.101 h(-1), 0.252 g l(-1), and 0.011 g MeOH g(-1) DCW h(-1), respectively. The unstructured model was validated in methanol induction phase with different initial cell densities. Results showed that the maximum specific protein production rate (q(p,max)) of 0.098 mg g(-1) DCW h(-1) was achieved when mu was kept at 0.016 h(-1), and the maximum yield of PIP reached 0.97 g l(-1), which was 1.5-fold as that of the control. Therefore, the simple Monod model proposed has proven to be a robust control system for recombinant porcine insulin precursor production by P. pastoris on pilot scale, which would be further applied on production scale.