文献类型：期刊文献

英文题名：Multiplex imaging of amyloid-β plaques dynamics in living brains with quinoline-malononitrile-based probes

作者：Dai, Jianfeng[1,2,3];Wei, Weijun[4];Yan, Chenxu[1,2];Ji, Ding-Kun[4];Liu, Caiqi[1,2];Huang, Jialiang[1,2];Liang, Chenyi[4];Liu, Jianjun[4];Guo, Zhiqian[1,2,3];Zhu, Wei-Hong[1,2,5]

机构：[1]East China Univ Sci & Technol, Inst Fine Chem, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Key Lab Adv Mat Feringa,Nobel, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Joint Int Res Lab Precis Chem & Mol Engn, Inst Fine Chem,Sch Chem & Mol Engn,Feringa Nobel P, Shanghai, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[4]Shanghai Jiao Tong Univ, Renji Hosp, Inst Clin Nucl Med, Dept Nucl Med,Sch Med, Shanghai, Peoples R China;[5]East China Univ Sci & Technol, Ctr Photosensit Chem Engn, Shanghai, Peoples R China

年份：2025

卷号：9

期号：10

起止页码：1632

外文期刊名：NATURE BIOMEDICAL ENGINEERING

收录：;EI(收录号：20252018420888);WOS:【SCI-EXPANDED(收录号:WOS:001494212100001)】；

基金：This work was supported by the National Key Research and Development Program (2021YFA0910000 to W.-H.Z.), NSFC/China (22225805, 32394001, 32121005, T2488302, 92356301, 22338006 and 22378122 to Z.G., Z.G., Z.G., W.-H.Z., W.-H.Z., W.-H.Z. and C.Y., respectively), Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission, grant 2021 Sci & Tech 03-28 to Z.G.), Science and Technology Commission of Shanghai Municipality (24DX1400200 to Z.G.), Shanghai Science and Technology Committee (23J21901600 and 23ZR1416600 to Z.G. and C.Y., respectively). We thank H.C. (Shanghai Institute of Materia Medica) and Z.A. (Shanghai Institute of Materia Medica) for allowing us to use the Photometrics Prime 95B sCMOS camera in dynamic in vivo fluorescence imaging.

语种：英文

外文关键词：Computerized tomography - Fluorescence microscopy - Neurodegenerative diseases

摘要：The dynamic behaviour of amyloid-beta (A beta) plaques in Alzheimer's disease remains poorly understood, and accumulation and distribution of A beta plaques must be inferred from in vitro pathological changes in brain tissue. In situ detection of A beta plaques in live imaging is challenging because of the lack of adequate probes. Here we report the design of unimolecular quinoline-malononitrile-based A beta probes, termed QMFluor integrative framework, that binds in vivo to A beta plaques, making them detectable via near-infrared fluorescence imaging, magnetic resonance imaging, positron emission tomography and computed tomography. QMFluor probes are permeable to the blood-brain barrier, and, upon systematic injection, enable real-time magnetic resonance imaging and positron emission tomography-computed tomography imaging of the A beta biodistribution in the hippocampus and cerebral cortex, and accurately differentiate the brains of living Alzheimer's disease mouse models from wild-type controls. We further demonstrate the ability of QMFluor probes to reach the brain after intravenous injection in a large animal model. This strategy expands the toolbox of probes for in vivo visualization of amyloids in Alzheimer's disease pathological analysis, drug screening and clinical applications.