文献类型：期刊文献

英文题名：Fluid dynamics investigation of variant impeller combinations by simulation and fermentation experiment

作者：Xia, Jian-Ye[1];Wang, Yong-Hong[1];Zhang, Si-Liang[1];Chen, Ning[1];Yin, Peng[1];Zhuang, Ying-Ping[1];Chu, Ju[1]

机构：[1]E China Univ Chem Technol, Coll Biotechnol, State Key Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2009

卷号：43

期号：3

起止页码：252

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20090411877141);WOS:【SCI-EXPANDED(收录号:WOS:000263617600005)】；

基金：Financial support from the National Natural Science Foundation of China (20476028), National Basic Research Program (973 Program no. 2007CB714303), and Shanghai Leading Academic Discipline Project (B505) is gratefully acknowledged.

语种：英文

外文关键词：Dissolved oxygen; Fluid mechanics; Gas-liquid mass transfer; Mixing; Mycelia fermentation; Shear environment

摘要：Fluid dynamics in three bioreactors equipped with different impeller combinations were investigated by Computational Fluid Dynamics (CFD) modeling, and avermectin fermentation experiments by Streptomyces avermitilis were also conducted in these bioreactors. Down-pumping propeller (DPP), 6-curved-blade disc turbine (6CBDT) and 6-arrowy-blade disc turbine (6ABDT) were combined to form different impeller combinations, which were tested in this work. The results of simulation showed that impeller combination consisting of upper two DPP and one bottom 6CBDT could provide the modest flow field environment for avermectin fermentation among the tested impeller combinations. Mass transfer study showed that even if the same impellers were used, different impeller combinations gave different K(L)a value. It was shown that same "radial flow impeller" such as 6ABDT or 6CBDT used in this work would produce the same shear force in the impeller sweeping zone, but it was different for the "axial flow impeller" Such as DPP used in this work, which produced different forces at different mounting positions. The relationship between the fluid dynamics and the fungal cell physiological situation was also discussed based on results of simulation and experiments. (C) 2008 Elsevier B.V. All rights reserved.