文献类型：期刊文献

英文题名：Enhancing cofactor regeneration of cyanobacteria for the light-powered synthesis of chiral alcohols

作者：Fan, Jianhua[1,2];Zhang, Yinghui[1,2];Wu, Ping[1,2];Zhang, Xiaoyan[1,3];Bai, Yunpeng[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Dept Appl Biol, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Dept Bioengn, Shanghai 200237, Peoples R China

年份：2022

卷号：118

外文期刊名：BIOORGANIC CHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000821662200004)】；

基金：This work was sponsored by National Key Research and Development Project of China (2019YFA0906300 and 2020YFA0907304), Natural Science Foundation of China (22078096), Natural Science Foundation of Shandong Province (ZR2019ZD17), Natural Science Foundation of Shanghai (21ZR1416400), Shanghai Pujiang Program 18PJD008, and Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Cyanobacteria; Biocatalysis; Asymmetric synthesis; Chiral alcohol; Cofactor regeneration

摘要：Cyanobacteria Synechocystis sp. PCC 6803 was exploited as green cell factory for light-powered asymmetric synthesis of aromatic chiral alcohols. The effect of temperature, light, substrate and cell concentration on substrate conversions were investigated. Under the optimal condition, a series of chiral alcohols were synthesized with conversions up to 95% and enantiomer excess (ee) > 99%. We found that the addition of Na2S2O3 and Angeli's Salt increased the NADPH content by 20% and 25%, respectively. As a result, the time to reach 95% substrate conversion was shortened by 12 h, which demonstrated that the NADPH regeneration and hence the reaction rates can be regulated in cyanobacteria. This blue-green algae based biocatalysis showed its potential for chiral compounds production in future.