文献类型：期刊文献

中文题名：克鲁斯假丝酵母分批发酵生产甘油的代谢流分布

英文题名：Metabolic Flux Distribution in Batch Cultivation of Candida krusei for Glycerol Production

作者：唐军[1];蔡水洪[2];叶勤[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室;[2]华东理工大学化学工程研究所,上海200237

年份：2002

卷号：16

期号：1

起止页码：58

中文期刊名：高校化学工程学报

外文期刊名：Journal of Chemical Engineering of Chinese Universities

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家"九五"科技攻关项目(96-C03-03-03)

语种：中文

中文关键词：Candidakrusei;分批发酵;代谢流分布;甘油;发酵法生产;克鲁斯假丝酵母;碳硫平衡法

外文关键词：Candida krusei; batch culture; metabolic flux analysis

摘要：采用Candida krusei 分批发酵生产甘油,在碳元素平衡基础上作代谢流分析得知,典型生长阶段(6～12h)形成菌体流量较大,占50%以上,且有12%的乙醇流量,甘油流量较小;典型生产阶段(48～54h)甘油流量增大为42%, 菌体和乙醇流量为0, 有11%的有机酸流量.
Analysis of metabolic flux distribution in fermentation is favorable to understanding the efficiency of the process. In this study, glycerol fermentation was carried out in a computer-controlled 15L-fermentor, and on-line data including oxygen and carbon dioxide contents in the exit gas were collected in the computer. A complex medium containing glucose, corn steep liquor, and urea was used. Ethanol was the main by-product during the cell growth phase, while gluconate and pyruvate were accumulated during the glycerol production phase. Carbon balance revealed that more than 96% of the carbon in the medium was recovered in the cell and measured metabolites, and the contribution of both corn steep liquor and glucose to cell growth was considered. According to the specific rates of cell growth, glucose consumption and metabolites formation, the metabolic flux distribution was estimated by 17 stoichiometrical equations. Although the specific carbon dioxide evolution rate was not used as a constraint, the calculated specific evolution rate was consistent with the measured one. During the typical growth phase (6~12h), carbon fluxes to monomers of the cellular structural macromolecules and that to ethanol were 53.8% and 11.9% respectively based on the flux of glucose uptake, while that to glycerol was only 2.1%. During the typical production phase (48~54h), the cells stopped growing, and the carbon flux to glycerol was increased to 42% and those to organic acids accounted for 11%. These results suggested the ways to improve glycerol fermentation, that is, suppression of ethanol formation during the growth phase and limitation of the formation of fatty acids during the production phase.