文献类型：期刊文献

英文题名：Rapid and high-throughput purification of salvianolic acid B from Salvia miltiorrhiza Bunge by high-performance counter-current chromatography

作者：Zhang, Min[1,2];Ignatova, Svetlana[3];Liang, Qionglin[1];Jun, Frank Wu[4];Sutherland, Ian[3];Wang, Yiming[1];Luo, Guoan[1,2]

机构：[1]Tsinghua Univ, Inst Biomed, Beijing 100084, Peoples R China;[2]E China Univ Sci & Technol, Modern Engn Ctr Tradit Chinese Med, Sch Pharm, Shanghai 200237, Peoples R China;[3]Brunel Univ, Brunel Inst Bioengn, Uxbridge UB8 3PH, Middx, England;[4]XiangCam TCM Res Ctr Ltd, Unit 16, Cambridge CB22 3AT, England

年份：2009

卷号：1216

期号：18

起止页码：3869

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000265699900025)】；

基金：This study was funded by Xiangxue Pharmaceuticals Group, China and sponsored by the National Basic Research Program (973 Program) of China (2007CB714505, 2005CB523503) and the National Key Technology R&D Program of China (2006BA108BO4-01). Also the authors would like to thank colleagues at Brunei Institute for Bioengineering, UK-Dr. Hugh Guan, Dr. Ian Garrard, Professor Derek Fisher, Dr. Remco van den Heuvel, Mr. Peter Hewitson, Mr. Joost de Folter; at East China University of Science and Technology-Dr. Ping Hu; at Tsinghua University, China-Mr. Xiaocheng Qi, Mr. Yijun Cheng; at Sichuan University, China-Mr. Haoyu Ye for their help and support during experimental work. S.I. and I.S. greatly appreciate the financial support provided by HEIF3 and West Focus to fund their supervision of this work.

语种：英文

外文关键词：Counter-current chromatography; Salvianolic acid B; Salvia miltiorrhiza Bunge; High throughput; Process efficiency

摘要：A large-scale purification of salvianolic acid B from Salvia miltiorrhiza Bunge is presented. The method development began with selection of the solvent system, then optimization of the operating parameters and ended up with linear scale-up from an analytical to a preparative instrument. Three factors were used for method optimization and scale-up estimation: purity, process throughput and process efficiency. Preparation was achieved using a two-phase solvent system comprising hexane-ethyl acetate-methanol-acetic acid-water (1:5:1.5:0.00596:5, v/v). This preparation yielded 475 mg of salvianolic acid B with a purity of 96.1% from 1.5g of crude extract. The process throughput of crude was 2.23 g/h while process efficiency per gram of target compound was 0.769 g/h. Two factors-process environmental risk factor and process evaluation factor were used for evaluation of the separation process. (C) 2009 Elsevier B.V. All rights reserved.