文献类型：期刊文献

英文题名：Novel surfactant peptide for removal of biofilms

作者：Sun, Wen[1];Wang, Yibing[1];Zhang, Wenxue[1];Ying, Hanjie[2];Wang, Ping[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai Collaborat Innovat Ctr Biomfg Technol, Sch Biotechnol,Biomed Nanotechnol Ctr, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Nanjing Tech Univ, State Key Lab Mat Oriented Chem Engn, Coll Biotechnol & Pharmaceut Engn, 30 Puzhu South Rd, Nanjing 211816, Jiangsu, Peoples R China;[3]Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA

年份：2018

卷号：172

起止页码：180

外文期刊名：COLLOIDS AND SURFACES B-BIOINTERFACES

收录：;EI(收录号：20183505747579);WOS:【SCI-EXPANDED(收录号:WOS:000455858500022)】；

基金：This work was supported by the National Natural Science Foundation of China (31471659, 21636003 and 21303050).

语种：英文

外文关键词：Affinity binding; Surfactant peptide; Biosurfactant; Escherichia coil O157:H7; Biofilm

摘要：Conventional chemical surfactants attach on blots randomly, accompanied with health and environmental issues. To address this, a surfactant peptide was designed to mimic chemical surfactants with an affinity binding peptide as a hydrophobic tail for the cleanup of biofilm contaminations. The micelle forming and structural changes of the peptide in aqueous solution were systematically investigated. More importantly, the biofilm removal efficiency toward Escherichia coli O157:H7 biofilm reached 75% in neutral aqueous solutions at the concentration of 125 mg/L (critical micelle concentration 91 mg/L), a significant improvement in comparison to conventional surfactants and random surfactant peptide. The dynamic removal process reported by confocal laser scanning microscope (CLSM) also displayed the different constituents of biofilm blots, which associated with surfactant peptide binding efficiency. Hopefully, this surfactant strategy will eventually provide new scopes in the design of surface active biological agents.