文献类型：期刊文献

英文题名：Biodegradable macroporous scaffold with nano-crystal surface microstructure for highly effective osteogenesis and vascularization

作者：Chu, Linyang[1];Jiang, Guoqiang[2];Hu, Xi-Le[3,4];James, Tony D.[5];He, Xiao-Peng[3,4];Li, Yaping[2];Tang, Tingting[1]

机构：[1]Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Shanghai Key Lab Orthoped Implants,Dept Orthoped, Shanghai 200011, Peoples R China;[2]Ningbo Univ, Affiliated Hosp, Sch Med, Dept Orthopaed Surg, Ningbo 315211, Zhejiang, Peoples R China;[3]East China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Shanghai 200237, Peoples R China;[5]Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England

年份：2018

卷号：6

期号：11

起止页码：1658

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;EI(收录号：20181204920303);WOS:【SCI-EXPANDED(收录号:WOS:000428186200011)】；

基金：This research was financially supported by the National Key R&D Program (2016YFC1102100), Ningbo Municipal Natural Science Foundation (2010C500009) and Zhejiang Natural Science Foundation (2017LY17H060001). X.-P. He thanks the Natural Science Foundation of China (21722801 and 21572058) and the Shanghai Rising-Star Program (16QA1401400) (to X.-P. He) for generous financial support. The Catalysis And Sensing for our Environment (CASE) network is thanked for research exchange opportunities. T. D. J. wishes to thank the Royal Society for a Wolfson Research Merit Award and ECUST for a guest professorship.

语种：英文

外文关键词：Mammals - Gene expression - Adhesion - Scaffolds (biology) - Bone - Calcium - Microstructure

摘要：Using the hydrothermal calcination method, bovine cancellous bone was transformed into a degradable macroporous scaffold with a nano-crystal surface microstructure, capable of releasing bioactive ions. Compared with the control group, the presence of the nano-crystal microstructure of the material scaffold significantly promoted the gene expression of adhesion proteins including integrin and vinculin, thus facilitating attachment, spreading, proliferation and focal adhesion formation of MC3T3-E1 cells on the surface of the scaffold. Additionally, the release of active magnesium and calcium ions from the scaffold promoted expression of osteogenic genes and formation of calcium nodules in osteoblasts. Both in vitro and in vivo assays demonstrated that the three-dimensional interconnected porous architecture promoted vascularization and tissue integration. Our findings provide new insight into the development of degradable macroporous composite materials with three-dimensional surface microstructures as bone substitutes or tissue engineering scaffolds with potential for clinical applications.