文献类型：期刊文献

英文题名：Display of adenoregulin with a novel Pichia pastoris cell surface display system

作者：Ren, Ren[1]; Jiang, Zhengbing[1]; Liu, Meiyun[1]; Tao, Xinyi[1]; Ma, Yushu[1]; Wei, Dongzhi[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China

年份：2007

卷号：35

期号：2

起止页码：103

外文期刊名：Molecular Biotechnology

收录：EI(收录号：20071710574458)

语种：英文

外文关键词：Amino acids - Cells - Confocal microscopy - Enzyme kinetics - Flocculation - Yeast

摘要：Two Pichia pastoris cell surface display vectors were constructed. The vectors consisted of the flocculation functional domain of Flo1p with its own secretion signal sequence or the α-factor secretion signal sequence, a polyhistidine (6xHis) tag for detection, an enterokinase recognition site, and the insertion sites for target proteins. Adenoregulin (ADR) is a 33-amino-acid antimicrobial peptide isolated from Phyllomedusa bicolor skin. The ADR was expressed and displayed on the Pichia pastoris KM71 cell surface with the system reported. The displayed recombinant ADR fusion protein was detected by fluorescence microscopy and confocal laser scanning microscopy (CLSM). The antimicrobial activity of the recombinant adenoregulin was detected after proteolytic cleavage of the fusion protein on cell surface. The validity of the Pichia pastoris cell surface display vectors was proved by the displayed ADR. ? 2006 Humana Press Inc. All rights of any nature whatsoever reserved.