文献类型：期刊文献

英文题名：A Self-Immobilizing and Fluorogenic Probe for -Lactamase Detection

作者：Mao, Wuyu[1];Xia, Lingying[1];Wang, Yaqun[1];Xie, Hexin[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2016

卷号：11

期号：24

起止页码：3493

外文期刊名：CHEMISTRY-AN ASIAN JOURNAL

收录：;EI(收录号：20165003112678);WOS:【SCI-EXPANDED(收录号:WOS:000392511500004)】；

基金：This research is financially supported by the National Natural Science Foundation of China (21472048), the "Thousand Plan" Youth Program, the Fundamental Research Funds for the Central Universities and East China University of Science & Technology. We thank Prof. Jianghong Rao at Stanford University for kindly providing TEM-1 bla plasmid.

语种：英文

外文关键词：antibiotic resistance; enzymes; fluorescent probes; immobilization; -lactamase

摘要：The spread of antibiotic resistance in pathogenic bacteria has become one of the major concerns to public health. Improved monitoring of drug resistance is of high importance for infectious disease control. One of the major mechanisms for bacteria to overcome treatment of antibiotics is the production of -lactamases, which are enzymes that hydrolyze the -lactam ring of the antibiotic. In this study, we have developed a self-immobilizing and fluorogenic probe for the detection of -lactamase activity. This fluorogenic reagent, upon activation by -lactamases, turns on a fluorescence signal and, more importantly, generates a covalent linkage to the target enzymes or the nearby proteins. The covalent labeling of enzymes was confirmed by SDS-PAGE analysis and MALDI-TOF mass spectrometry. The utility of this structurally simple probe was further confirmed by the fluorescent labeling of a range of -lactamase-expressing bacteria.