文献类型：期刊文献

英文题名：Optimization of fermentation conditions for peptides from waste jasmine flower and its anti-aging effect on H2O2-induced normal human dermal fibroblasts

作者：Wu, Mengqi[1,2];Wei, Xinxin[1];Feng, Hongyu[1];Liu, Yikang[1];Xia, Wei[1];Zhang, Wenqing[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China

年份：2024

卷号：218

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20242416234999);WOS:【SCI-EXPANDED(收录号:WOS:001256851600001)】；

基金：This research was funded by "Science and Technology Innovation Action Plan" for Shanghai Municipal Science and Technology Support Project (Grant No. 22S21902800) . The authors also thank Kunming Nuofa Biotechnology Co., Ltd. for kindly providing waste jasmine flower after scenting process in the jasmine tea production.

语种：英文

外文关键词：Peptides; Lactobacillus; Waste jasmine flower

摘要：In the process of refining jasmine essential oil, a significant amount of flower residue is produced, which contains valuable bioactive substances. However, this waste is not adequately utilized. This study aimed to ferment jasmine residue to extract low molecular peptides for the development of high -value products. A Box-Behnken design was used to optimize the fermentation conditions. The results revealed that an inoculation amount of 8.2 %, a fermentation temperature of 39 degrees C, and a fermentation time of 47.7 h produced the highest content of peptides, which increased by 39.2 % compared to the non -fermented samples. Subsequently, three peptides named JSFP 1 -1, JSFP 5 -1, and JSFP 5 -3 were purified using ion exchange resin and gel column chromatography and their amino acid sequences were determined using UPLC-QTOF-MS/MS. Furthermore, their anti -aging activity was investigated. The results indicated that the peptides promoted cell proliferation and COL -1 secretion, while significantly reducing SA- beta-gal expression, intracellular ROS level and MMP-1 secretion in the H 2 O 2 - induced human dermal fibroblast (HDF) cell senescence model. These findings suggest the potential of jasmine residue fermentation products as cosmetic raw materials with anti -aging properties and provide a solid theoretical foundation for their development in the cosmetic industry.