文献类型：期刊文献

英文题名：Degradation of chlorophenols catalyzed by laccase

作者：Zhang, Jianbo[1];Liu, Xiaopeng[1];Xu, Zhenqiang[1];Chen, Hui[1];Yang, Yuxiang[2]

机构：[1]Peking Univ, Coll Environm Sci, Dept Environm Sci, Beijing 100871, Peoples R China;[2]E China Univ Sci & Technol, Coll Chem & Pharmaceut, Shanghai 200237, Peoples R China

年份：2008

卷号：61

期号：4

起止页码：351

外文期刊名：INTERNATIONAL BIODETERIORATION & BIODEGRADATION

收录：;EI(收录号：20082111271555);WOS:【SCI-EXPANDED(收录号:WOS:000257003200010)】；

语种：英文

外文关键词：chlorophenol; laccase; catalytic degradation; immobilized enzyme

摘要：The degradations of 2,4-dichloroplienol (2,4-DCP), 4-chlorophenol (4-CP) and 2-chlorophenol (2-CP) catalyzed by laccase were carried out. The optimal condition regarding degradation efficiency was also discussed, which included reaction time, pH value, temperature, concentration series of chlorophenols and laccase. Results showed that the capability of laccase was the best, while to oxidize 2,4-DCPamong the above-mentioned chlorophenols. Within 10 h, file removal efficiency of 2,4-DCP, 2-CP and 4-CP could reach 94%, 75% and 69%, respectively. The optimal pH for laccase to degrade chlorophenols was around 5.5. The increase of laccase concentration or temperature might result in the degradation promotion. The trends of degradation percentage were various among these three chlorophenols with the concentration increase of chlorophenols. Degradation of 2,4-DCP is a first-order reaction and the reaction activation energy is about 44.8 kJ mol(-1). When laccase was immobilized on chitosan, crosslinked with glutaraldehyde, the activity of immobilized laccase was lower than that of free laccase, but the stability improved significantly. The removal efficiency of immobilized laccase to 2,4-DCP still remained over 65% after six cycles of operation. (C) 2007 Elsevier Ltd. All rights reserved.