文献类型：期刊文献

中文题名：连续二维开管离子交换/反相整体柱毛细管电色谱的构建与评价

英文题名：Construction and Evaluation of Continuous Two-dimensional Open-tubular Ion Exchange/Reversed Phase Monolithic Column Capillary Electrochromatography System

作者：朱亚仙[1];张凌怡[1];雷雯[1];张维冰[1]

机构：[1]华东理工大学上海市功能性材料化学重点实验室,上海200237

年份：2011

卷号：32

期号：1

起止页码：44

中文期刊名：高等学校化学学报

外文期刊名：Chemical Journal of Chinese Universities

收录：CSTPCD;;Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000287008700010)】；北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家"九七三"计划项目(批准号:2007CB914100);国家自然科学基金(批准号:21075039)资助

语种：中文

中文关键词：毛细管电色谱;二维分离;整体柱

外文关键词：Capillary electrochromatography; Two-dimensional separation; Monolithic column

摘要：在毛细管中原位合成反相整体色谱柱,并在同一根毛细管柱中的其余部分通过在内表面涂覆N-[3-(三甲氧基硅烷)-丙基)]-乙二胺(PEDA)使其具有离子交换功能,制备成连续二维开管离子交换/反相整体柱毛细管电色谱柱.通过对7种有机酸的分离探讨了开管柱中离子交换对分离的影响,进一步以天麻提取物为样品,对二维分离系统加以评价,出峰数量达到34个.在构建的新型电色谱柱系统中,可以通过电渗流、电泳流以及溶质与固定相的作用同时对分离选择性加以调节,适用于强疏水性和强亲水性同时存在的复杂样品的分析方法的建立.
A continuous two-dimensional open-tubular ion exchange/reversed phase monolithic column capillary electrochromatography system was developed.Reversed phase monolithic column was firstly synthesized in the end part of the capillary,then N-[3-（trimethoxysilyl）-propyl]-ethlenediamine was surface coated to the other part of the capillary.The separation of seven organic acids verified the effect on the separation of ion exchange in the open-tubular capillary.Rhizoma gastrodiae was separated to evaluate the system,and 34 peaks were recognized.This system is designed for complex samples with highly hydrophobicity components and highly hydrophilicity components simultaneous.This new column system combining advantages of multidimensional separation and adjusted stationary phase selectivity is suitable for the analysis method development of complicated samples.