文献类型：期刊文献

英文题名：Kinetics and equilibria of the chromatographic separation of maltose and trehalose

作者：He, Dengjun[1,2];Zhou, Jiachun[1];Xia, Quanming[1];Jiang, Lihua[1];Qiu, Yongjun[1,2];Zhao, Liming[1,2]

机构：[1]E China Univ Sci & Technol, R&D Ctr Separat & Extract Technol Fermentat Ind, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol SC, Shanghai, Peoples R China

年份：2015

卷号：38

期号：13

起止页码：2229

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20152100867653);WOS:【SCI-EXPANDED(收录号:WOS:000357695100005)】；

基金：This work is financially supported by the National Natural Science Foundation of China (NO. 31371725) and National High Technology Research and Development Program of China (SS2014AA021202).

语种：英文

外文关键词：Adsorption isotherm; Axial dispersion; Mass transfer; Maltose; Trehalose

摘要：Trehalose, a nonreducing disaccharide, has been extensively applied to food, cosmetics, and pharmaceutical goods. The resultant solution of trehalose prepared by enzymatic methods includes high amounts of maltose. However, it is quite difficult to separate maltose and trehalose on an industrial scale because of their similar properties. In this paper, a high-performance resin was selected as a stationary phase to separate trehalose and maltose, and the resolution of these sugars was 0.59. The potential of a cation exchange resin was investigated as the stationary phase in separating trehalose and maltose using deionized water as the mobile phase. Based on the equilibrium dispersive model, the axial dispersion coefficients and overall mass transfer coefficients of maltose and trehalose were determined by moment analysis at two different temperatures, 50 and 70 degrees C. Other parameters, including the column void and the adsorption isotherms, were also determined and applied to simulate the elution curves of trehalose and maltose. The simulated results matched the experimental data, validating the parameters. The optimized parameters are critical to the chromatographic separation of trehalose and maltose on an industrial scale.