文献类型：期刊文献

英文题名：A cost-effective Z-folding controlled liquid handling microfluidic paper analysis device for pathogen detection via ATP quantification

作者：Jin, Sheng-Quan[1];Guo, Su-Miao[1];Zuo, Peng[1];Ye, Bang-Ce[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China

年份：2015

卷号：63

起止页码：379

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20150400449901);WOS:【SCI-EXPANDED(收录号:WOS:000343337000052)】；

基金：This study was supported by the National Natural Science Foundation of China (Nos. 31101285 and 21335003), the Key Grant Project (No. 313019) of the Chinese Ministry of Education, and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Microfluidic paper analysis device; ATP detection; Pathogen detection; Liquid control

摘要：A cost-effective microfluidic paper analysis device (mu PAD) was developed with a special Z-folding design for controlling the fluidic flowing and substrate transportation. This presented mu PAD can be easily fabricated through wax printing by using a solid ink printer which deposits wax onto the surface of a chromatographic paper, and then baked on a hotplate by penetrating the molten wax into the paper to create a hydrophobic barrier. After mu PAD fabrication, liquid control and substrate transportation can be easily carried out by twice folding the mu PAD following Z shape. The Z folding made two separated reagent holding zone connected while the detection reaction occurred with the connection. In this paper, a pathogens detection indicated by ATP quantification was took as a proof-in-principle application of using this presented mu PAD, the limit of detection (LOD) was 1 mu M for ATP detection and 2.6 x 10(7) CFU/mL for Salmonella live cell detection, which showed a great potential for Point-of-Care Testing (POCT) applications. (C) 2014 Elsevier B.V. All rights reserved.