文献类型：期刊文献

英文题名：Multi-channel colorimetric and fluorescent probes for differentiating between cysteine and glutathione/homocysteine

作者：Song, Lun[1];Jia, Ti[2,3];Lu, Wenjia[1];Jia, Nengqin[2,3];Zhang, Weibing[1,4];Qian, Junhong[1,4]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Educ Minist, Key Lab Resource Chem, Shanghai 200234, Peoples R China;[3]Coll Life & Environm Sci, Dept Chem, Shanghai Key Lab Rare Earth Funct Mat, Shanghai 200234, Peoples R China;[4]Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Peoples R China

年份：2014

卷号：12

期号：42

起止页码：8422

外文期刊名：ORGANIC & BIOMOLECULAR CHEMISTRY

收录：;EI(收录号：20144300123179);WOS:【SCI-EXPANDED(收录号:WOS:000343956000009)】；

基金：This work was financially supported by the National 973 Program (no. 2011CB910403), NSFC (no. 21235005) and the Open Project Program of State Key Laboratory of Food Science and Technology, Nanchang University (no. SKLF-KF-201215). We thank Prof. Y. Yang (East China University of Science & Technology) for his kind help.

语种：英文

外文关键词：Peptides - Fluorescence spectroscopy - Probes - Fluorescence

摘要：Three fluorescent probes TP1-3 for thiols were rationally designed and synthesized to distinguish cysteine (Cys) from glutathione (GSH)/homocysteine (Hey). TP1-3 are almost non-fluorescent and colorless 4-nitro-1,8-naphthalimide derivatives. Upon the substitution of nitro by Cys, TP1-3 were transformed into weakly fluorescent green-emitting 4-amino analogs via highly fluorescent blue-emitting thioether intermediates. The three-channel signaling capability allows discrimination between Cys and GSH/Hcy. The fluorescence intensity at 498 nm was linearly proportional to GSH concentration in the range of 0-20 mu M, and the detection limit was 5 x 10(-8) mol L-1. A good linear relationship between A(446)/A(350) and Cys concentration was found in the range of 0-70 mu M, and the detection limit was 2 x 10(-7) ml L-1. Moreover, TP3 was used for living cell imaging as well as for detecting mercapto-containing proteins.