文献类型：期刊文献

英文题名：Improvement of lincomycin production by mutant selection and metabolic regulation

作者：Pang, Xuewei[1];Zheng, Yitao[1];Qiao, Xianting[1];Mao, Quangui[2];Ma, Qian[2];Ye, Ruifang[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Topfond Pharmaceut Co Ltd, Zhengzhou, Henan, Peoples R China

年份：2017

卷号：47

期号：7

起止页码：639

外文期刊名：PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000407899900001)】；

语种：英文

外文关键词：Bioreactor; lincomycin; mutant; propylproline; two-dimensional gel electrophoresis

摘要：Lincomycin is a lincosamide antibiotic produced by Streptomyces lincolnensis. Through mutagenesis by ethylmethansulfonate (EMS) and ultraviolet (UV) irradiation repeatedly, M2 was picked out in plate with glutamine and propylproline orderly. In 50-L stirred bioreactor, the production of lincomycin, fermented by M2, was increased to 8136u/ml under the optimal condition as compared to original strain S. lincolnensis 07-5 (6634u/ml). Two-dimensional gel electrophoresis (2-D GE) and mass spectrometry (MS)-shown LmbG, LmbI, and acetohydroxy acid isomeroreductase were remarkably synthesized in M2. The gene lmbG and lmbI are responsible for methylation in the lincomycin biosynthetic cluster, while acetohydroxy acid isomeroreductase contributes to stronger metabolic capability. Finally, we obtained a better strain for industrial production.