文献类型：期刊文献

英文题名：Dual-function injectable fibrin gel incorporated with sulfated chitosan nanoparticles for rhBMP-2-induced bone regeneration

作者：Chen, Xinye[1,3];Wang, Shuang[2];Zhang, Xiaohui[2];Yu, Yuanman[1,3];Wang, Jing[1,3];Liu, Changsheng[2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Shanghai 200237, Peoples R China

年份：2022

卷号：26

外文期刊名：APPLIED MATERIALS TODAY

收录：;EI(收录号：20220411492248);WOS:【SCI-EXPANDED(收录号:WOS:000784043100008)】；

基金：The authors wish to express their gratitude to the financial supports from the National Natural Science Foundation of China for Innovative Research Groups (No. 51621002) and the National Natural Science Foundation of China (No. 31870953) . This study was also supported by the Weigao Project of Chinese Academy of Science [2020] 005.

语种：英文

外文关键词：BMP-2; Sulfated chitosan; Immunomodulation; Bone regeneration

摘要：A B S T R A C T Bone fractures commonly occur in the aging patients, and exhibit slow fracture healing rates and high complication rates even with the best treatment strategies available. Additionally, aging patients always suffer from immunosenescence, and thus induce the dysfunction of macrophages to delay the bone repair. Although exogenous bone morphogenetic protein-2 (BMP-2) is verified that can efficiently promote bone regeneration, the supraphysiologic usage is always associated with abnormal heterotopic ossification and inflammatory response. Here, we have developed a dual-function injectable fibrin gel (Fg) with semisynthetic sulfated chitosan (SCS) nanoparticles (NPs) that significantly improves the recombinant human BMP-2 (rhBMP-2)-induced osteogenesis and down-regulates the inflammatory response at the fracture site. The prepared fibrinogen and sulfated chitosan nanoparticles (SCS NPs) efficiently prolong the delivery of rhBMP-2 and decrease the volume of bone callus. Moreover, SCS incorporated Fg regulates the macrophage polarization from pro-inflammatory M1 macrophage to anti-inflammatory M2 macrophage with a significant decrease of inflammatory-related cytokines. Finally, this dual-function injectable gel can also efficiently treat bone fractures well in postmenopausal mice with osteoporosis. Thus, this proosteogenic gel offers a translational potential for biomaterial-based bone healing. (c) 2021 Elsevier Ltd. All rights reserved.