文献类型：专利

英文题名：New recombinant plasmid pBBR-PtufB-Vitreoscilla hemoglobin, useful e.g. for expressing Vitreoscilla hemoglobin in the growth process, formed by the Vitreoscilla hemoglobin gene, the PtufB promoter of the Gluconobacter oxydans and the vector

作者：WU J;LI M;WEI D;LIN J

机构：[1]UNIV EAST CHINA SCI&TECHNOLOGY

申请号：CN102041264-A

申请日：2009-10-10

公开日：2011-05-04

语种：英文

收录：DERWENT

摘要：NOVELTY - Recombinant plasmid pBBR-PtufB-Vitreoscilla hemoglobin (VHb) formed by the Vitreoscilla hemoglobin gene, the PtufB promoter of the Gluconobacter oxydans and the vector, is new. USE - The genetically engineered bacteria of Gluconobacter oxydans is useful for expressing Vitreoscilla hemoglobin in the growth process and for converting the glycerol into 1,3-dihydroxyacetone (all claimed) and also for intracellular expression of Vitreoscilla hemoglobin and increasing the catalytic cell biomass. ADVANTAGE - The genetically engineered bacteria is utilized for producing 1,3-dihydroxyacetone to maintain the existing conditions and energy consumption under same pressure, and thus it solves the inadequate oxygen supply of the Gluconobacter oxydans in the cell culture and catalytic process. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: (1) constructing recombinant plasmid pBBR-PtufB-VHb; (2) a genetically engineered bacteria of Gluconobacter oxydans, obtained by converting the recombinant plasmid pBBR-PtufB-VHb into the Gluconobacter oxydans; and (3) constructing genetically engineered bacteria of Gluconobacter oxydans, comprising: (i) amplifying the Vitreoscilla hemoglobin gene, ligating into the suitable vector to obtain the plasmid pBBR-VHb; (ii) amplifying the PtufB promoter of the Gluconobacter oxydans, ligating into the plasmid pBBR-VHb to obtain the recombinant plasmid pBBR-PtufB-VHb; and (iii) converting the recombinant plasmid pBBR-PtufB-VHb into the Gluconobacter oxydans to obtain the genetically engineered bacteria of Gluconobacter oxydans.